MC1R, a Melanocortin Receptor, mediates the effects of melanocyte-stimulating hormone (MSH) and adrenocorticotropic hormone (ACTH). MSH and its receptor, MC1R, are key regulators of melanogenesis. They control the relative proportions of red pheomelanin and black photoprotective eumelanin in the skin by increasing the production of eumelanin. Non-functional, truncated forms of the receptor lead to lighter coat color in animals. In contrast, constitutively active receptors lead to darker color. Pheomelanin generates free radicals in response to UV radiation, and elevated levels of pheomelanin have been associated with higher risk of UV-induced skin damage. Therefore, MC1R is a major determinant of sun sensitivity and genetic risk for melanoma and other skin cancers. Expression of melanocortin 1 receptor has been reported primarily in adrenal, skin, and testis. ESTs have been isolated from breast, placenta, and testis libraries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Details: Human lymphocytes were isolated from PBMCs. Lymphocytes were blocked with Natural Goat Serum for 15 minutes, and then stained and subsequently incubated at -20 C with anti-human MC1R primary antibody for 30 minutes. After washing, cells were stained with Alexa Fluor 488 Donkey anti-rabbit IgG and incubated at -20 C for 40 minutes. After washing, cells were detected using a Fortessa 4-15 flow cytometer.
Product General Protocols
View specific protocols for Melanocortin-1 R/MC1R Antibody (NLS1040):
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