MEL-18 Antibody - BSA Free

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Western Blot: MEL-18 Antibody [NBP2-04032] - Whole cell lysate from HeLa (15 and 50 ug), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibody used at 0.1 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

MEL-18 Antibody - BSA Free Summary

Description
Novus Biologicals Rabbit MEL-18 Antibody - BSA Free (NBP2-04032) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Immunogen
The immunogen recognized by this antibody maps to a region between residue 125 and 175 of human DNA-binding Protein Mel-18 using the numbering given in entry NP_009075.1 (GeneID 7703).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PCGF2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MEL-18 Antibody - BSA Free

  • MEL-18
  • polycomb group ring finger 2
  • polycomb group RING finger protein 2
  • RING finger protein 110DNA-binding protein Mel-18
  • RNF110MEL18
  • Zinc finger protein 144
  • ZNF144MGC10545

Background

MEL-18 is a transcriptional repressor that binds specifically to the DNA sequence 5'-GACTNGACT-3'. Mel18 has tumor suppressor activity and may play a role in cell proliferation and/or neural cell development. It regulates proliferation of early T progenitor cells by maintaining expression of HES1. Also plays a role in antero-posterior specification of the axial skeleton and negative regulation of the self-renewal activity of hematopoietic stem cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PCGF2
Entrez