MEK2 Antibody (300317) [DyLight 550]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: 300317
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 550

MEK2 Antibody (300317) [DyLight 550] Summary

• Immunogen: E. coli-derived recombinant human MEK2
  Met1-Val400
  Accession # P36507
• Specificity: Detects human, mouse, and rat MEK2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) MEK1 or rhMEK5 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAP2K2
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for MEK2 Antibody (300317) [DyLight 550]

• ERK activator kinase 2
• MAP2K2
• MAPKK 2
• MEK 2
• MEK2
• MEK2EC 2.7.12.2
• mitogen-activated protein kinase kinase 2
• MKK2
• MKK2FLJ26075
• p45
• PRKMK2MAPKK2

Background

MEK1 and MEK2 (MAPK kinase 1/2, or ERK kinase 1/2) are mitogen-activated protein kinases that stimulate MAP kinase activity, playing a role in both cell growth and differentiation (1,2). MEK itself is activated via phosphorylation at serines 217/218 and 221/222 by upstream activator kinases, including c-raf, mos and MEK kinase (3,4). MEK1 and MEK2 are activated by a wide variety of growth factors and cytokines, and also by membrane depolarization and calcium influx (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAP2K2