MED7 Antibody - BSA Free

Western Blot: MED7 Antibody [NBP2-17258] - Sample (30 ug of whole cell lysate) A: Hela 12% SDS PAGE gel, diluted at 1:1000.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MED7 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MED7 Antibody - BSA Free (NBP2-17258) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MED7. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MED7
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for MED7 Antibody - BSA Free

• Activator-recruited cofactor 34 kDa component
• Cofactor required for Sp1 transcriptional activation subunit 9
• cofactor required for Sp1 transcriptional activation, subunit 9 (33kD)
• cofactor required for Sp1 transcriptional activation, subunit 9, 33kDa
• CRSP33CRSP complex subunit 9
• CRSP9hMED7
• mediator complex subunit 7ARC34
• mediator of RNA polymerase II transcription subunit 7
• MGC12284
• RNA polymerase transcriptional regulation mediator subunit 7 homolog
• Transcriptional coactivator CRSP33

Background

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: MED7
• Uniprot:
  • O43513(Human)