MED21 Antibody (2D9)

Western Blot: MED21 Antibody (2D9) [H00009412-M10] - Analysis of SURB7 expression in HeLa (Cat # L013V1).

Immunocytochemistry/Immunofluorescence: MED21 Antibody (2D9) [H00009412-M10] - Analysis of monoclonal antibody to SURB7 on HeLa cell. Antibody concentration 10 ug/ml

ELISA: MED21 Antibody (2D9) [H00009412-M10] - Detection limit for recombinant GST tagged SURB7 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 2D9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

MED21 Antibody (2D9) Summary

• Immunogen: SURB7 (AAH08380, 1 a.a. ~ 144 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MADRLTQLQDAVNSLADQFCNAIGVLQQCGPPASFNNIQTAINKDQPANPTEEYAQLFAALIARTAKDIDVLIDSLPSEESTAALQAASLYKLEEENHEAATCLEDVVYRGDMLLEKIQSALADIAQSQLKTRSGTHSQSLPDS
• Specificity: SURB7 (2D9)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MED21
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MED21 Antibody (2D9)

• hSrb7
• mediator complex subunit 21SRB7 (suppressor of RNA polymerase B, yeast) homolog
• RNA polymerase II complex component SRB7
• RNA polymerase II holoenzyme component SRB7
• RNAPII complex component SRB7
• SRB7 suppressor of RNA polymerase B homolog
• SRB7SRB7 suppressor of RNA polymerase B homolog (yeast)
• SURB7mediator of RNA polymerase II transcription subunit 21

Background

MED21 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymera

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MED21
• Entrez:
  • 9412(Human)
• OMIM:
  • 603800(Human)
• Uniprot:
  • AAH08380(Human)