MDM2/HDM2 Antibody (SMP14) [PE]

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IP
• Clone: SMP14
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PE

MDM2/HDM2 Antibody (SMP14) [PE] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Synthetic peptide C-SRPSTSSRRRAISE, corresponding to amino acids 154-167 of human MDM2/HDM2. [UniProt# Q00987]
• Epitope: Amino acids 154 - 167.
• Localization: Nuclear and cytoplasmic (expressed predominantly in the nucleoplasm).
• Specificity: The SMP14 monoclonal antibody also recognises a peptide epitope around Thr-216 of murine MDM2 (when Thr-216 is unphosphorylated) see Zhang & Prives, 2001 (PMID: 11359766) for further details. SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MDM2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Immunohistochemistry - Frozen and Paraffin, Immunoprecipitation and Western blot. This antibody has also been reported useful in IHC on resin tissue sections. Staining of formalin-fixed paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
  
  Recommended applications are based on validated applications from the unconjugated base product NB100-2736. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 15625077). Mouse reactivity reported in scientific literature (PMID: 25981963).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for MDM2/HDM2 Antibody (SMP14) [PE]

• ACTFS
• HDM2
• HDMX
• MDM2 oncogene, E3 ubiquitin protein ligase
• MDM2

Background

MDM2 is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MDM2