MCM5 Antibody (CRCT5.1) - BSA Free

Immunohistochemistry-Paraffin: MCM5 Antibody (CRCT5.1) [NB100-65643] - Staining of formalin fixed, paraffin embedded human tonsil with Mouse anti Humann MCM5.

• Reactivity: Hu
• Applications: IHC, IHC-P
• Clone: CRCT5.1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

MCM5 Antibody (CRCT5.1) - BSA Free Summary

• Immunogen: Bacterially expressed human Mcm5
• Specificity: NB100-65643 recognizes human Mcm-5 (minichromosome maintenance protein 5), a protein with an important role in the control of DNA replication. It has been reported that immunocytochemical assessment of Mcm5 expression may be of value in improving the accuracy of cervical smear testing for the detection of malignancy.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MCM5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:1000
  • Immunohistochemistry-Paraffin 1:50-1: 1000
• Application Notes: Immunohistochemistry-Paraffin This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for MCM5 Antibody (CRCT5.1) - BSA Free

• CDC46 homolog
• CDC46DNA replication licensing factor MCM5
• EC 3.6.4.12
• MCM5 minichromosome maintenance deficient 5, cell division cycle 46 (S.cerevisiae)
• MCM5 minichromosome maintenance deficient 5, cell division cycle 46
• MGC5315
• minichromosome maintenance complex component 5
• minichromosome maintenance deficient (S. cerevisiae) 5 (cell division cycle 46)
• minichromosome maintenance deficient 5 (cell division cycle 46)
• P1-CDC46

Background

MCM5 (Mini Chromosome Maintenance protein-5) is involved in the control of DNA replication. MCM proteins have DNA dependent ATPase motifs in their central domain which is conserved from yeast to mammals. MCM proteins form a complex which exhibits ATPase and helicase activities. In G0 phase levels of MCM 2 and 5 are lower than that of MCM7 and 3 but increase as the cell enters G1/S phase of the cell cycle. It has been reported that immunocytochemical assessment of Mcm5 expression may be of value in improving the accuracy of cervical smear testing for the detection of malignancy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MCM5
• Entrez:
  • 4174(Human)
• Uniprot:
  • P33992(Mouse)