MCM3 Antibody (JB99-35)

Images

 
Western Blot: MCM3 Antibody (JB99-35) [NBP2-75569] - Analysis of MCM3 on mouse thymus tissue (1), NIH-3T3 cell (2) and Hela (3)cell lysates using anti-MCM3 antibody at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence: MCM3 Antibody (JB99-35) [NBP2-75569] - Staining MCM3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: MCM3 Antibody (JB99-35) [NBP2-75569] - Analysis of paraffin-embedded rat testis tissue using anti-MCM3 antibody. Counter stained with hematoxylin.
Flow Cytometry: MCM3 Antibody (JB99-35) [NBP2-75569] - Analysis of K562 cells with MCM3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa ...read more
Immunocytochemistry/ Immunofluorescence: MCM3 Antibody (JB99-35) [NBP2-75569] - Staining MCM3 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunocytochemistry/ Immunofluorescence: MCM3 Antibody (JB99-35) [NBP2-75569] - Staining MCM3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: MCM3 Antibody (JB99-35) [NBP2-75569] - Analysis of paraffin-embedded human tonsil tissue using anti-MCM3 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: MCM3 Antibody (JB99-35) [NBP2-75569] - Analysis of paraffin-embedded human placenta tissue using anti-MCM3 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: MCM3 Antibody (JB99-35) [NBP2-75569] - Analysis of paraffin-embedded mouse small intestine tissue using anti-MCM3 antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
JB99-35
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

MCM3 Antibody (JB99-35) Summary

Immunogen
Recombinant protein corresponding to human MCM3 aa 1-200
Localization
Nucleus.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
MCM3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin
Theoretical MW
91 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for MCM3 Antibody (JB99-35)

  • cervical cancer proto-oncogene 5
  • DNA polymerase alpha holoenzyme-associated protein P1
  • DNA replication factor MCM3
  • DNA replication licensing factor MCM3
  • EC 3.6.4.12
  • HCC5
  • hRlf beta subunit
  • MCM3 minichromosome maintenance deficient 3 (S. cerevisiae)
  • MCM3 minichromosome maintenance deficient 3
  • MGC1157
  • minichromosome maintenance complex component 3
  • minichromosome maintenance deficient (S. cerevisiae) 3
  • minichromosome maintenance deficient 3
  • P1.h
  • p102
  • P1-MCM3
  • replication licensing factor, beta subunit
  • RLF subunit beta
  • RLFB

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MCM3