MAT1/2A Antibody - BSA Free

Western Blot: MAT1/2A Antibody [NB110-94162] - Detection of MAT2A in HepG2 whole cell lysates using NB110-94162.

Simple Western: MAT1/2A Antibody [NB110-94162] - Simple Western lane view shows a specific band for MAT1/2 A in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Rt, Bv, Pm, Ze, Mu(-)
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

MAT1/2A Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MAT1/2A Antibody - BSA Free (NB110-94162) is a polyclonal antibody validated for use in WB and Simple Western. Anti-MAT1/2A Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide made to an internal portion of the human MAT2A protein (within residues 100-200). [Swiss-Prot# P31153]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAT2A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Simple Western 1:10
  • Western Blot 2 ug/ml
• Application Notes: This MAT1/2A antibody is useful for Western blot, where a band is seen at approx. 43 kDa.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HepG2 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:10, apparent MW was 74 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  MAT2A Overexpression Lysate

• Control Peptide:

  MAT1/2A Peptide (NB110-94162PEP)

Reactivity Notes

Orangutan. Does not react with mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MAT1/2A Antibody - BSA Free

• AdoMet synthase 2
• AMS2
• EC 2.5.1.6
• MAT 2
• MATA2SAMS2adoMet synthetase 2
• MATII
• MAT-II
• Methionine adenosyltransferase 2
• Methionine adenosyltransferase II
• methionine adenosyltransferase II, alpha
• S-adenosylmethionine synthase isoform type-2
• S-adenosylmethionine synthetase isoform type-2

Background

MAT1A and MAT2A are catalytic subunits of methionine adenosyltransferase (MAT), which catalyzes the formation of S-adenosyltransferase for methionine catabolism. Inactivation of MAT1A has been linked to liver disease. In addition, in certain liver cancers expression of MAT1A is replaced by expression of MAT1B. Overexpression of MAT2A has also been observed in colon cancer, leading to the proposal that MAT2A facilitates cancer cell growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MAT1/2A Antibody (NB110-94162)(2)

Publications using NB110-94162

• Hung CY, Zhu C, Kittur FS et al. A plant-based mutant huntingtin model-driven discovery of impaired expression of GTPCH and DHFR Cellular and molecular life sciences : CMLS 2022-10-17 [PMID: 36251090] (WB, Mouse)
• Navik U, Sheth VG, Kabeer SW, Tikoo K Dietary Supplementation of Methyl Donor L-Methionine Alters Epigenetic Modification in Type 2 Diabetes Mol Nutr Food Res 2019-09-18 [PMID: 31532875]

Review for MAT1/2A Antibody (NB110-94162) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Steve Smith WB Human 12/14/2014

Summary

• Application: Western Blot
• Sample Tested: Whole cell lysate prepared from RT-4 cells
• Species: Human

Bioinformatics

• Gene Symbol: MAT2A