MAT1/2A Antibody

Images

 
Western Blot: MAT1/2A Antibody [NB110-94162] - Detection of MAT2A in HepG2 whole cell lysates using NB110-94162.
Simple Western: MAT1/2A Antibody [NB110-94162] - Simple Western lane view shows a specific band for MAT1/2 A in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Rt, Bv, Pm, Ze, Mu(-)Species Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

MAT1/2A Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of the human MAT2A protein (within residues 100-200). [Swiss-Prot# P31153]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAT2A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Simple Western 1:10
Application Notes
This MAT1/2A antibody is useful for Western blot, where a band is seen at approx. 43 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
MAT2A Overexpression Lysate
Control Peptide
MAT1/2A Peptide (NB110-94162PEP)
Reviewed Applications
Read 1 Review rated 5
using
NB110-94162 in the following applications:

Publications
Read Publication using NB110-94162.

Reactivity Notes

Human, rat, orangutan, bovine, Zebrafish and monkey. Does not react with mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MAT1/2A Antibody

  • AdoMet synthase 2
  • AMS2
  • EC 2.5.1.6
  • MAT 2
  • MATA2SAMS2adoMet synthetase 2
  • MATII
  • MAT-II
  • Methionine adenosyltransferase 2
  • Methionine adenosyltransferase II
  • methionine adenosyltransferase II, alpha
  • S-adenosylmethionine synthase isoform type-2
  • S-adenosylmethionine synthetase isoform type-2

Background

MAT1A and MAT2A are catalytic subunits of methionine adenosyltransferase (MAT), which catalyzes the formation of S-adenosyltransferase for methionine catabolism. Inactivation of MAT1A has been linked to liver disease. In addition, in certain liver cancers expression of MAT1A is replaced by expression of MAT1B. Overexpression of MAT2A has also been observed in colon cancer, leading to the proposal that MAT2A facilitates cancer cell growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-94162
Species: Hu, Rt, Bv, Pm, Ze, Mu(-)
Applications: WB, Simple Western

Publications for MAT1/2A Antibody (NB110-94162)(1)

Review for MAT1/2A Antibody (NB110-94162) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB110-94162:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot MAT1/2A NB110-94162
Enlarge
5
reviewed by:
Steve Smith
WB Human 12/14/2014
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate prepared from RT-4 cells
SpeciesHuman

Blocking

Blocking DetailsPVDF was blocked with 5% milk in PBS buffer

Primary Anitbody

Dilution RatioPrimary antibody diluted 500 times in PBS buffer

Secondary Antibody

Secondary DescriptionBioRad goat anti-rabbit HRP
Secondary Manufacturer Cat#170-5046
Secondary Concentration20000 times diluted

Details

Detection NotesDeveloped using BioRad WesternC Chemiluminescence Kit, 1min exposure

Product General Protocols

View specific protocols for MAT1/2A Antibody (NB110-94162): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MAT1/2A Antibody (NB110-94162) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-94162AF350
Alexa Fluor 405 NB110-94162AF405
Alexa Fluor 488 NB110-94162AF488
Alexa Fluor 532 NB110-94162AF532
Alexa Fluor 594 NB110-94162AF594
Alexa Fluor 647 NB110-94162AF647
Alexa Fluor 700 NB110-94162AF700
Alexa Fluor 750 NB110-94162AF750
Biotin NB110-94162B
DyLight 350 NB110-94162UV
DyLight 405 NB110-94162V
DyLight 488 NB110-94162G
DyLight 550 NB110-94162R
DyLight 594 NB110-94162DL594
DyLight 650 NB110-94162C
DyLight 680 NB110-94162FR
DyLight 755 NB110-94162IR
HRP NB110-94162H
Janelia Fluor 549 NB110-94162JF549
Janelia Fluor 646 NB110-94162JF646

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Bioinformatics Tool for MAT1/2A Antibody (NB110-94162)

Discover related pathways, diseases and genes to MAT1/2A Antibody (NB110-94162). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Calculators

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Steve Smith
12/14/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol MAT2A