Mast Cell Protease-11/Prss34 Antibody (451421)

• Reactivity: Mu
• Applications: WB, IP
• Clone: 451421
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Mast Cell Protease-11/Prss34 Antibody (451421) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Mast Cell Protease‑11/Prss34
  Met20-Ser318
  Accession # Q80UR4
• Specificity: Detects mouse Mast Cell Protease‑11/Prss34 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) MCP-1, rmMCP-6, or rmMCP-7 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: Prss34
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mast Cell Protease-11/Prss34 Antibody (451421)

• EOS
• Mast Cell Protease11
• Mast Cell Protease-11
• MCP-11
• MCPT11
• Prss34

Background

Mast Cell Protease-11 (MCP-11) is encoded by Prss34, one of 13 genes on mouse chromosome 17A3.3 that correspond to functional trypsin-like serine proteases (1). The deduced amino acid sequence of mouse MCP-11 consists of 318 residues with a signal peptide (residues 1 to 19), a pro region (residue 20 to 34), and a catalytic domain (35 to 318). The mRNA is preferentially expressed in spleen and bone marrow. The amino acid sequence of mouse MCP-11 shares 84% and 60% identity with that of rat and dog, respectively. The human gene corresponding to Prss34 contains a mutation that leads to a premature translation termination codon, thus encoding a protein that is unlikely to be enzymatically active.

1. Wong, G.W. et al. (2004) J. Biol. Chem. 279:2438.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Prss34
• Uniprot:
  • Q80UR4()