Western Blot: MARCKS [p Ser152, p Ser156] Antibody [NB500-140] - Western blot of rat brain lysate showing specific immunolabeling of the ~87k MARCKS protein phosphorylated at Ser152,156 (Control). The phosphospecificity ...read more
Rt, Ca, ChHa, Hu, Mu, Bv, Ch, Xp, ZeSpecies Glossary
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser152/156 conjugated to KLH
p Ser152, p Ser156
Cytoplasmic; actin cytoskeleton
Specific for the ~87k MARCKS protein phosphorylated at Ser152 and Ser156 in Western blots. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. The immunolabeling is completely eliminated by lambda-phosphatase
Human (100%), Mouse (100%), Zebrafish (100%), Bovine (100%), Chicken (100%), Xenopus (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
NB500-140 can be used in Western blot where a band is seen at ~ 80 kDa, representing MARCKS. Immunohistochemical analysis has not yet been determined.
87 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Alternate Names for MARCKS [p Ser152, p Ser156] Antibody
MACSmyristoylated alanine-rich C-kinase substrate
myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L)
myristoylated alanine-rich protein kinase C substrate
Protein kinase C substrate, 80 kDa protein, light chain
Myristolated Alanine-Rich C-Kinase Substrate (MARCKS) is a major in vivo substrate of protein kinase C (PKC) and for the lipid-activated PKC-related kinase (PRK1). Furthermore, PRK1 and OPKC phosphorylate MARCKS on the same sites in vitro, serine 152, 156, and 163. MARCKS serves as an in vitro substrate for PKCu as well. However, in contrast to other PKCs, MARCKS is phosphorylated by PKCu only at serine 156 and not at serines 152 and 163, implicating a differential regulation by PKCu. Therefore, control of MARCKS phosphorylation on these previously identified 'PKC' sites may be regulated under certain circumstances by PRK as well as PKC mediated signaling pathways. MARCKS associates with the plasma membrane in response to PKC-catalyzed phosphorylation of MARCKS. It has been suggested that MARCKS is capable of associating with the plasma membrane through binding to phospholipids without interaction with membranous proteins.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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