MAPKAPK2 Antibody (7H4.2) - BSA Free

Western Blot: MAPKAPK2 Antibody (7H4.2) [NBP2-29767] - Lysates from Hela were resolved by electrophoresis, transferred to PVDF and probed with anti-MAPKAPK2 (1 ug/mL). Proteins were visualized probed with using goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates phosphorylated MAPKAPK2 (45 kDa).

• Reactivity: Hu, Mu, Rt
• Applications: WB, IP
• Clone: 7H4.2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

MAPKAPK2 Antibody (7H4.2) - BSA Free Summary

• Description: Relevant Entrez Gene Numbers: NM_004759.3;NM_032960.2
• Immunogen: KLH-conjugated synthetic peptide of the C-terminus of the long form of human MAPKAP-K2.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAPKAPK2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:20
  • Western Blot 1:100-1:2000
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.2 mg antibody with PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Notes

Relevant Entrez Gene Numbers: NM_004759.3;NM_032960.2

Alternate Names for MAPKAPK2 Antibody (7H4.2) - BSA Free

• EC 2.7.11
• EC 2.7.11.1
• MAP kinase-activated protein kinase 2
• MAPK-activated protein kinase 2
• MAPKAP kinase 2
• MAPKAPK2
• MAPKAPK-2
• mitogen-activated protein kinase-activated protein kinase 2
• MK2
• Rps6kc1

Background

MAPKAP Kinase 2 encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPKAPK2
• Uniprot:
  • P49137(Human)