MAP1 Antibody

Western Blot: MAP1 Antibody [NBP1-02988] - MAP-1 in EL4 cell lysate with MAP-1 antibody (IN) at (A) 1, (B) 2, and (C) 4 ug/ml.

Immunohistochemistry-Paraffin: MAP1 Antibody [NBP1-02988] - Staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Product Discontinued

MAP1 Antibody Summary

• Immunogen: A 15 amino acid synthetic peptide near the center of human MAP1. The immunogen is located within the last 50 amino acids of MAP1.
• Epitope: Near the center
• Specificity: 15 amino acid peptide from near the center of human MAP1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MOAP1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for MAP1 Antibody

• MAP1
• MAP-1PNMA4Paraneoplastic antigen Ma4
• modulator of apoptosis 1
• paraneoplastic antigen like 4

Background

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Members of the Bcl-2 family are known to be critical regulators of this process. These proteins are characterized by the presence of several conserved motifs termed Bcl-2 homology (BH) domains. A related protein termed MAP-1 has recently been identified. This protein contains a BH3-like domain and induces caspase-dependent apoptosis in mammalian cells when overexpressed. It forms homodimers and associates with Bcl-2 family members such as Bax, Bcl-2, and Bcl-XL in vitro and in vivo. It has been suggested that MAP-1 associates with the tumor suppressor RASSF1A following death receptor activation, allowing a conformational change in Bax that leads to cellular apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for MAP1 Antibody (NBP1-02988). (Showing 1 - 1 of 1 FAQ).

1. I have checked in your website that the antibody NBP1-02988 works with human and mouse and it can be used in IHC-P for human brain tissue. I would like to enquire if this antibody can be used in IF for mouse brain tissue? If not, could you please advise me on which of your products can be used in this application?
   • The antibody you are inquiring about, NBP1-02988, has not been validated for immunoflourescence in any species. I did look for another MOAP1 antibody that would be more suitable for IF but cannot find one. If you would be interested in testing this antibody in IF (new application) I invite you to read more about our Innovators Reward Program.

Bioinformatics

• Gene Symbol: MOAP1
• Entrez:
  • 64112(Human)
  • 64113(Mouse)
• Uniprot:
  • Q96BY2(Human)