Recombinant Human MANF Protein

Product Discontinued

Recombinant Human MANF Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 25-182 of Human ARMET
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMLRPGD CEVCISYLGR FYQDLKDRDV TFSPATIENE LIKFCREARG KENRLCYYIG ATDDAATKII NEVSKPLAHH IPVEKICEKL KKKDSQICEL KYDKQIDLST VDLKKLRVKE LKKILDDWGE TCKGCAEKSD YIRKINELMP KYAPKAASAR TDL

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: MANF
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 20.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol, 0.1 M NaCl
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human MANF Protein

• Arginine-Rich Protein
• arginine-rich, mutated in early stage tumors
• ARMET
• ARP
• MANF
• mesencephalic astrocyte-derived neurotrophic factor
• Protein ARMET

Background

Mesencephalic astrocyte-derived neurotrophic factor, also known as MANF, is a 20 kDa member of the ARMET family of proteins. MANF was initially identified as a protein containing an arginine-rich region that was highly mutated in a variety of tumors. Expression of MANF has also been shown to be induced during ER stress, suggesting that it may play a role in protein quality control during ER stress. Recombinant human MANF protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: MANF