Human MANF ELISA Kit (Colorimetric)

ELISA: Human MANF ELISA Kit (Colorimetric) [NBP2-81157] - Samples were spiked with high concentrations of Human MANF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human MANF ELISA Kit (Colorimetric) [NBP2-81157] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL

Human MANF ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human MANF in samples. No significant cross-reactivity or interference between Human MANF and analogues was observed.
• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: %CV < 5.66
• Intra-Assay: %CV < 4.46
• Spike Recovery: 85 - 112%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: MANF

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human MANF ELISA Kit (Colorimetric)

• Arginine-Rich Protein
• arginine-rich, mutated in early stage tumors
• ARMET
• ARP
• MANF
• mesencephalic astrocyte-derived neurotrophic factor
• Protein ARMET

Background

MANF is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and promotes cell proliferation. The protein was initially thought to be longer at the N-terminus and to contain an arginine-rich region but transcribed evidence indicates a smaller open reading frame that does not encode the arginine tract. The presence of a specific mutation changing the previously numbered codon 50 from ATG to AGG, or deletion of that codon, has been reported in a variety of solid tumors. With the protein size correction, this codon is now identified as the initiation codon. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: MANF