MANF Antibody

Product Discontinued

MANF Antibody Summary

• Immunogen: Peptide with sequence KYAPKAASAPTDL, from C-Terminus of the protein sequence according to NP_006001
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MANF
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA 1:32000
  • Western Blot 1:100-1:2000
• Application Notes: Western Blot: Approx 30kDa band seen in HeLa cell lysate. Recommended for use at 2-4ug/ml.
• Control:

  HeLa Whole Cell Lysate
  MANF Overexpression Lysate

Reactivity Notes

Cross-reacts with Human. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MANF Antibody

• Arginine-Rich Protein
• arginine-rich, mutated in early stage tumors
• ARMET
• ARP
• MANF
• mesencephalic astrocyte-derived neurotrophic factor
• Protein ARMET

Background

Armet encodes a highly conserved protein whose function is not yet known. The protein was initially thought to be longer at the N-terminus and to contain an arginine-rich region but transcribed evidence indicates a smaller open reading frame that does not encode the arginine tract. The presence of a specific mutation changing the previously numbered codon 50 from ATG to AGG, or deletion of that codon, has been reported in a variety of solid tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MANF
• Entrez:
  • 7873(Human)
• Uniprot:
  • NP_006001(Human)