MAGEA1 Antibody (9Y6K1)

Western Blot: MAGE 1 Antibody (9Y6K1) [NBP3-15301] - Western blot analysis of extracts of various cell lines, using MAGE 1 Rabbit mAb (NBP3-15301) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clone: 9Y6K1
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

MAGEA1 Antibody (9Y6K1) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-309 of human MAGEA1 (P43355). VMIAMEGGHAPEEEIWEELSVMEVYDGREHSAYGEPRKLLTQDLVQEKYLEYRQVPDSDPARYEFLWGPRALAETSYVKVLEYVIKVSARVRFFFPSLREAALREEEEGV
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MAGEA1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for MAGEA1 Antibody (9Y6K1)

• Antigen MZ2-E
• Cancer/testis antigen 1.1
• cancer/testis antigen family 1, member 1
• CT1.1
• CT1.1melanoma antigen family A 1
• MAGE-1 antigen
• MAGE1
• MAGE1A
• MAGE1melanoma antigen MAGE-1
• MAGEA1
• MAGE-A1
• melanoma antigen family A, 1 (directs expression of antigen MZ2-E)
• melanoma-associated antigen 1
• melanoma-associated antigen MZ2-E
• MGC9326
• MZ2-E

Background

The MAGE 1 gene is a member of the melanoma antigen encoding gene family. These genes encode for HLA-restricted tumor associated rejection antigens recognized by cytotoxic T lymphocytes. Some of these target antigens may be potentially useful for cancer specific immunotherapy. The expression of MAGE genes has been reported not only in melanoma but also in various other malignant tumors such as hepatocellular carcinoma and germ cell tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAGEA1