MafB Antibody (BLR046F)

Western Blot: MafB Antibody [NBP3-14673] - Antibody: Rabbit anti-MafB recombinant monoclonal antibody used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgGChemiluminescence with an exposure time of 3 minutes.Lower Panels: Rabbit anti-MafA recombinant monoclonalantibody and rabbit anti-Actinrecombinant monoclonal antibody.

Immunohistochemistry: MafB Antibody [NBP3-14673] - Rabbit anti-MafBrecombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Western Blot: MafB Antibody [NBP3-14673] - Whole cell lysate (50 ug) from HeLa, HEK293T, SK-N-MC, RPMI-8226, Jurkat, KG-1, K-562, and MOLT-4 cells prepared using NETN lysis buffer. Rabbit anti-MafB recombinant monoclonal antibody [BLR046F] (NBP3-14673 lot 2) used at 1:1000. HRP-conjugated goat anti-rabbit IgG (NB7156). Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2.

Immunohistochemistry: MafB Antibody [NBP3-14673] - Rabbit anti-MafBrecombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: DAB.

Immunoprecipitation: MafB Antibody [NBP3-14673] - Samples: Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from SK-N-MC cellsprepared using NETN lysis buffer. Antibodies: Rabbit antiMafB recombinant monoclonal antibody used for IP at 6 ul/mg lysate. MafB wasalso immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated MafB, was used at 1:1000. Chemiluminescence withan exposure time of 30 seconds.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IP
• Clone: BLR046F
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

MafB Antibody (BLR046F) Summary

• Description: Novus Biologicals Rabbit MafB Antibody (BLR046F) (NBP3-14673) is a recombinant monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal
• Immunogen: between 1 and 50
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MAFB
• Purity: >95%

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100 to 1:500
  • Immunohistochemistry 1:100 to 1:500
  • Immunohistochemistry-Paraffin 1:100 to 1:500
  • Immunoprecipitation 6 ul/1 mg lysate
  • Western Blot 1:1000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Borate Buffered Saline (BBS) pH 8.2 with , BSA Free
• Preservative: 0.09% Sodium Azide
• Purity: >95%

Alternate Names for MafB Antibody (BLR046F)

• Kreisler (mouse) maf-related leucine zipper homolog
• Kreisler maf-related leucine zipper homolog
• KRML
• KRMLMAFB/Kreisler basic region/leucine zipper transcription factor
• MafB
• Maf-B
• MGC43127
• transcription factor MafB
• v-maf musculoaponeurotic fibrosarcoma oncogene homolog B (avian)
• V-maf musculoaponeurotic fibrosarcoma oncogene homolog B

Background

MAFB is encoded by this gene is a basic leucine zipper (bZIP) transcription factor that plays an important role in the regulation of lineage-specific hematopoiesis. The encoded nuclear protein represses ETS1-mediated transcription of erythroid-specific genes in myeloid cells. This gene contains no introns.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAFB