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MAdCAM-1 Antibody (MECA-367)

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Immunohistochemistry: MAdCAM-1 Antibody (MECA-367) [NBP1-28146] - Mouse intestine section was stained with Rat Anti-Mouse MAdCAM-1-UNLB followed by an HRP conjugated secondary antibody, DAB, and hematoxylin.. Using the FITC format of this antibody.
Immunohistochemistry: MAdCAM-1 Antibody (MECA-367) [NBP1-28146] - Mouse intestine section was stained with Rat Anti-Mouse MAdCAM-1-UNLB followed by an HRP conjugated secondary antibody, DAB, and hematoxylin.. Using the ...read more
Immunohistochemistry: MAdCAM-1 Antibody (MECA-367) [NBP1-28146] - Staining of BALB/c mouse intestine section with Biotin and FITC conjugated antibodies.. Using the FITC format of this antibody.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-28146
    • Availability
      Product Discontinued

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MAdCAM-1 Antibody (MECA-367) Summary

Immunogen
Endothelial cells isolated from BALB/c mesenteric and peripheral lymph nodes
Specificity
Murine MAdCAM-1 (Mr 58-88 kDa)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
MADCAM1
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Block/Neutralize
  • Flow Cytometry 1 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
Reviewed Applications
Read 1 Review rated 5
using
NBP1-28146 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.1M BBS (pH 8.2)
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for MAdCAM-1 Antibody (MECA-367)

  • hMAdCAM-1
  • MACAM1
  • MAdCAM1
  • MAdCAM-1
  • mucosal addressin cell adhesion molecule 1
  • mucosal addressin cell adhesion molecule-1
  • mucosal vascular addressin cell adhesion molecule 1

Background

Mucosal vascular addressin-1 (MAdCAM-1) is a type I transmembrane glycoprotein expressed at high levels on high endothelial venules (HEV) of Peyer's patches and mesenteric lymph nodes, and on flat walled venules within the gut lamina propria. It is also expressed on sinus-lining cells in the spleen. (1-3) The countereceptor or 'homing receptor' for MAdCAM-1 is alpha4beta7 integrin (also known as LPAM-1). MAdCAM-1 is also a facultative ligand for CD62L/L-selectin. (4, 5) The monoclonal antibody MECA-367 binds to the first domain of MAdCAM-1 and blocks MAdCAM-1-dependent binding in vitro and lymphocyte homing to Peyer's patch HEV in vivo. (1, 2, 4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for MAdCAM-1 Antibody (NBP1-28146) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-28146:
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IHC-Fr
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen MAdCAM-1 NBP1-28146
Enlarge 		5
reviewed by:
Verified Customer
 IHC-Fr Mouse 02/21/2020
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse Spleen
SpeciesMouse
LotH2618-M600

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Immunohistochemistry-Frozen MAdCAM-1 NBP1-28146
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 Verified Customer
02/21/2020
Application: IHC-Fr
Species: Mouse
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Bioinformatics

Gene Symbol MADCAM1