Recombinant Human MAD3 His Protein

SDS-Page: MAD3 Protein [NBP2-23219]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human MAD3 His Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-206 of Human MAD3
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMEPLASN IQVLLQAAEF LERREREAEH GYASLCPHRS PGPIHRRKKR PPQAPGAQDS GRSVHNELEK RRRAQLKRCL ERLKQQMPLG ADCARYTTLS LLRRARMHIQ KLEDQEQRAR QLKERLRSKQ QSLQRQLEQL RGLAGAAERE RLRADSLDSS GLSSERSDSD QEELEVDVES LVFGGEAELL RGFVAGQEHS YSHGGGAWL

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: MXD3
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 25.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human MAD3 His Protein

• bHLHc13
• Class C basic helix-loop-helix protein 13
• MAD3FLJ35523
• MAX dimerization protein 3
• Max dimerizer 3
• Max-associated protein 3
• Max-interacting transcriptional repressor MAD3
• MGC2383
• MYX

Background

MXD3 is a member of the Myc superfamily of basic helix-loop-helix leucine zipper transcriptional regulators. The protein forms a heterodimer with the cofactor MAX which binds specific E-box DNA motifs in the promoters of target genes and regulates their transcription. Disruption of the MAX-MXD3 complex is associated with uncontrolled cell proliferation and tumorigenesis. Transcript variants of this gene encoding different isoforms have been described. Recombinant human MXD3 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for MAD3 Protein (NBP2-23219)(1)

Publication using NBP2-23219

• Li J, Donangelo I, Abe K et al. Thyroid hormone treatment activates protective pathways in both in vivo and in vitro models of neuronal injury. Mol. Cell. Endocrinol. 2017-05-23 [PMID: 28549992]

Bioinformatics

• Gene Symbol: MXD3