MAD3 Antibody

Product Discontinued

MAD3 Antibody Summary

• Immunogen: Peptide with sequence C-QEHSYSHGGGAWL corresponding to C-Terminus according to NP_112590.1.
• Specificity: This antibody is expected to recognise isoform a (NP_112590.1) only.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MXD3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Peptide ELISA Detection limit 1:4000
• Application Notes: IF: An anonymous customer has reported successful usage on HeLa cells (methanol-fixed and 0.5% TX100-permeabilized), staining the cytoplasm. WB: No signal obtained yet but low background observed in NCI-H460, HepG2, human liver, human heart and human brain lysates at up to 3ug/ml. IHC: An anonymous customer has reported positive results in on human HeLa cells.
• Control:

  MAD3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MAD3 Antibody

• bHLHc13
• Class C basic helix-loop-helix protein 13
• MAD3FLJ35523
• MAX dimerization protein 3
• Max dimerizer 3
• Max-associated protein 3
• Max-interacting transcriptional repressor MAD3
• MGC2383
• MYX

Background

Binds a membrane serine threonine kinase receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MAD3 Antibody (NB100-1116)(1)

Publication using NB100-1116

• Hurlin PJ, Queva C, Koskinen PJ et al. Mad3 and Mad4: novel Max-interacting transcriptional repressors that suppress c-myc dependent transformation and are expressed during neural and epidermal differentiation. EMBO J 1995-11-15 [PMID: 8521822]

Bioinformatics

• Gene Symbol: MXD3
• Entrez:
  • 83463(Human)
• Uniprot:
  • NP_112590(Human)