MAB21L2 Overexpression Lysate

Images

 
Western Blot: MAB21L2 Overexpression Lysate (Adult Normal) [NBL1-12783] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAB21L2.
Western Blot: MAB21L2 Overexpression Lysate (Adult Normal) [NBL1-12783] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for MAB21L2

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

MAB21L2 Overexpression Lysate Summary

Description

MAB21L2 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC203414

Accession#: NM_006439

Protein Tag: C-MYC/DDK
Gene
MAB21L2

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
40.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MAB21L2 Overexpression Lysate

  • FLJ31103
  • mab-21 (C. elegans)-like 2
  • mab-21-like 2 (C. elegans)
  • protein mab-21-like 2

Background

This gene is similar to the C. elegans MAB-21 cell fate-determining gene, a downstream target of transforming growth factor-beta signaling. It is thought that this gene may be involved in neural development. The protein encoded by this gene is primarily nuclear, although some cytoplasmic localization has been observed. [provided by RefSeq]. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this RefSeq transcript is supported by transcript alignments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-19961
Species: Hu, Mu
Applications: WB
314-BP/CF
Species: Hu
Applications: BA, BA
NB300-750
Species: Fi, Hu, Pm, Mu, Pm, Rt, Sh
Applications: ICC/IF, IHC, IP, WB
423-F8
Species: Hu, Mu
Applications: BA
DCRP00
Species: Hu
Applications: ELISA
NBP2-61435
Species: Hu, Mu
Applications: IHC, IHC-P, WB
AF3364
Species: Hu
Applications: ICC, IHC, WB
NB100-56467
Species: Ca, Hu, Pm
Applications: WB
AF650
Species: Hu
Applications: CyTOF-ready, Flow, IHC, WB
302-B2/CF
Species: Hu
Applications: BA
AF1513
Species: Mu
Applications: CyTOF-ready, Flow, IHC, IP, Simple Western, WB
H00009242-M01
Species: Hu
Applications: ELISA, WB
NBP1-90003
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-85630
Species: Hu
Applications: IHC, IHC-P, WB
NBP3-04984
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
AF2039
Species: Hu
Applications: IHC, Simple Western, WB
NB100-1218
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, PEP-ELISA, WB
NBP2-24551
Species: Bv, Hu, Mu, Rt
Applications: IHC, IHC-P, WB

Publications for MAB21L2 Lysate (NBL1-12783) (0)

There are no publications for MAB21L2 Lysate (NBL1-12783).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for MAB21L2 Lysate (NBL1-12783) (0)

There are no reviews for MAB21L2 Lysate (NBL1-12783). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MAB21L2 Lysate (NBL1-12783) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional MAB21L2 Products

Bioinformatics Tool for MAB21L2 Lysate (NBL1-12783)

Discover related pathways, diseases and genes to MAB21L2 Lysate (NBL1-12783). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MAB21L2 Lysate (NBL1-12783)

Discover more about diseases related to MAB21L2 Lysate (NBL1-12783).
 

Pathways for MAB21L2 Lysate (NBL1-12783)

View related products by pathway.

Research Areas for MAB21L2 Lysate (NBL1-12783)

Find related products by research area.

Blogs on MAB21L2

There are no specific blogs for MAB21L2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MAB21L2 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol MAB21L2