Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: JARID1B Antibody [NBP1-97310] - Jarid1B / KDM5B antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry: JARID1B Antibody [NBP1-97310] - IHC analysis of JARID1B in human breast cancer xenograft using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.828 mg/ml

Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human JARID1B protein (between residues 800-850) [UniProt Q9UGL1]
Localization
Nucleus.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KDM5B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
Application Notes
This JARID1B antibody is useful for Flow Cytometry, Immunocytochemistryand paraffin embedded sections. In ICC/IF, staining of the nucleus was seen in HeLa cells. In paraffin, nuclear staining was observed in human breast cancer xenograft. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in western blot reported in scientific literature ( PMID 23354547)
Publications
Read Publications using
NBP1-97310 in the following applications:

  • 1 publication
  • 1 publication
  • WB
    1 publication

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.02% Sodium Azide
Concentration
0.828 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody

  • CT31
  • CT31FLJ10538
  • EC 1.14.11
  • EC 1.14.11.-
  • homolog 1A
  • Jarid1B
  • JARID1BFLJ16281
  • Jumonji, AT rich interactive domain 1B (RBP2-like)
  • jumonji, AT rich interactive domain 1B
  • KDM5B
  • Lysine (K)specific Demethylase 5B
  • Lysine (K)-specific Demethylase 5B
  • PLU-1
  • PLU-1FLJ12459
  • PLU1FLJ23670
  • PUT1
  • RBBP2H1
  • RBBP2H1AFLJ12491
  • RBP2-H1
  • Retinoblastoma-binding protein 2 homolog 1

Background

Jumonji AT-rich interactive domain 1B (JARID1B) also known as Jumonji/ARID domain-containing protein 1B, Lysine-specific demethylase 5B (KDM5B), Cancer/testis antigen 31 (CT31), Histone demethylase JARID1B, PLU-1, and Retinoblastoma-binding protein 2 homolog 1 (RBP2-H1), is a member of the JARID 1 family. The JARID 1 proteins (JARID1A, JARID1B, JARID1C and JARID1D) catalyze the demethylation of histones on lysine, and JARID1B specifically demethylates lysine 4 on Histone H3. JARID1B functions as a transcriptional co-repressor for FOXG1B and PAX9. In addition, JARID1B may play a considerable role in cancer research as it assists in the propagation of breast cancer cells by repressing the tumor suppressor genes BRCA1 and HOXA5. Conversely, JARID1B acts as a direct suppressor of melanoma. As result, the JARID1B protein is up regulated in breast cancer and down regulated in melanoma and glioblastomas. In general, JARID1B expression is ubiquitous; with the highest expression seen in testis. JARID1B is subject to post translational phosphorylation and has two known isoforms; isoform 1: 175.6kDa and isoform 2: 179.4kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (NBP1-97310)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: FLOW, IHC-P, WB.


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(1)
IHC-P
(1)
WB
(1)
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Product General Protocols

View specific protocols for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (NBP1-97310): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (NBP1-97310) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-97310AF405
Alexa Fluor 488 Labeled NBP1-97310AF488
Alexa Fluor 647 Labeled NBP1-97310AF647
Alexa Fluor 700 Labeled NBP1-97310AF700
Biotin Labeled NBP1-97310B
DyLight 405 Labeled NBP1-97310V
DyLight 488 Labeled NBP1-97310G
DyLight 550 Labeled NBP1-97310R
DyLight 650 Labeled NBP1-97310C
FITC Labeled NBP1-97310F
HRP Labeled NBP1-97310H
PE Labeled NBP1-97310PE

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Bioinformatics

Gene Symbol KDM5B
Entrez
Uniprot