Immunocytochemistry/ Immunofluorescence: JARID1B Antibody [NBP1-97310] - Jarid1B / KDM5B antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry: JARID1B Antibody [NBP1-97310] - IHC analysis of JARID1B in human breast cancer xenograft using DAB with hematoxylin counterstain.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.4)
0.02% Sodium Azide
Immunogen affinity purified
This JARID1B antibody is useful for Flow Cytometry, Immunocytochemistryand paraffin embedded sections. In ICC/IF, staining of the nucleus was seen in HeLa cells. In paraffin, nuclear staining was observed in human breast cancer xenograft. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in western blot reported in scientific literature ( PMID 23354547)
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Alternate Names for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody
Jumonji, AT rich interactive domain 1B (RBP2-like)
jumonji, AT rich interactive domain 1B
Lysine (K)specific Demethylase 5B
lysine (K)-specific demethylase 5B
Retinoblastoma-binding protein 2 homolog 1
Jumonji AT-rich interactive domain 1B (JARID1B) also known as Jumonji/ARID domain-containing protein 1B, Lysine-specific demethylase 5B (KDM5B), Cancer/testis antigen 31 (CT31), Histone demethylase JARID1B, PLU-1, and Retinoblastoma-binding protein 2 homolog 1 (RBP2-H1), is a member of the JARID 1 family. The JARID 1 proteins (JARID1A, JARID1B, JARID1C and JARID1D) catalyze the demethylation of histones on lysine, and JARID1B specifically demethylates lysine 4 on Histone H3. JARID1B functions as a transcriptional co-repressor for FOXG1B and PAX9. In addition, JARID1B may play a considerable role in cancer research as it assists in the propagation of breast cancer cells by repressing the tumor suppressor genes BRCA1 and HOXA5. Conversely, JARID1B acts as a direct suppressor of melanoma. As result, the JARID1B protein is up regulated in breast cancer and down regulated in melanoma and glioblastomas. In general, JARID1B expression is ubiquitous; with the highest expression seen in testis. JARID1B is subject to post translational phosphorylation and has two known isoforms; isoform 1: 175.6kDa and isoform 2: 179.4kDa.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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