Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody - BSA Free

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352] - Staining in human testis and skeletal muscle tissues . Corresponding KDM5B RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352] - Immunohistochemical staining of human skin shows moderate to strong nuclear positivity in epidermal cells.

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352] - Immunohistochemical staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts and Leydig cells.

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352] - Immunohistochemical staining of human tonsil shows moderate to strong nuclear positivity in lymphoid cells.

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352] - Immunohistochemical staining of human rectum shows moderate to strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352] - Staining of human skeletal muscle shows weak nuclear positivity in myocytes.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: DGINSLERKLKRRLEREGLSSERWERVKKMRTPKKKKIKLSHPKDMNNFKLERERSYELVRSAETHSLPSDTSYSEQEDSE
• Predicted Species: Rat (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KDM5B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Immunoprecipitation Reported in scientific literature (PMID: 25190814)
  • Western Blot Reported in scientific literature (PMID: 25190814)
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Protein (NBP1-84352PEP)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24802759).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody - BSA Free

• CT31
• CT31FLJ10538
• EC 1.14.11
• EC 1.14.11.-
• homolog 1A
• Jarid1B
• JARID1BFLJ16281
• Jumonji, AT rich interactive domain 1B (RBP2-like)
• jumonji, AT rich interactive domain 1B
• KDM5B
• Lysine (K)specific Demethylase 5B
• Lysine (K)-specific Demethylase 5B
• PLU-1
• PLU-1FLJ12459
• PLU1FLJ23670
• PUT1
• RBBP2H1
• RBBP2H1AFLJ12491
• RBP2-H1
• Retinoblastoma-binding protein 2 homolog 1

Background

JARID1B (Jumonji AT-rich interactive domain 1B) is a member of the JARID 1 family of proteins that catalyze the demethylation of histones on lysine. The Jarid 1 family members include JARID1A, JARID1B, JARID1C and JARID1D. The members of this family possess a Jumonji C (JmjC) and Jumonji N (JmjN) domain as well as one ARID (AT-rich interactive domain) and three PHD-type zinc fingers. As histone demethylases, JARID 1 proteins function as transcriptional repressors. JARID1B expression is largely restricted to testis and may play a critical role in tumorigenesis as it has been found to be upregulated in prostate and breast cancer. Alternative names for JARID1B include retinoblastoma-binding protein 2 homolog 1, RBP2-H1, cancer/testis antigen 31, CT31, PLU-1, PUT1, and KDM5B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (NBP1-84352)(10)

Publications using NBP1-84352

• Zhang SM, Cai WL, Liu X Et al. KDM5B promotes immune evasion by recruiting SETDB1 to silence retroelements Nature 2021-10-01 [PMID: 34671158] (Chemotaxis)
• Li G, Kanagasabai T, Lu W et al. KDM5B Is Essential for the Hyperactivation of PI3K/AKT Signaling in Prostate Tumorigenesis Cancer Research 2020-11-01 [PMID: 32868382]
• Wang L, Mao Y, Du G et al. Overexpression of JARID1B is associated with poor prognosis and chemotherapy resistance in epithelial ovarian cancer. Tumour Biol 2015-04-01 [PMID: 25663457] (IF/IHC, Human)
• Lu W, Liu S, Li B et al. SKP2 inactivation suppresses prostate tumorigenesis by mediating JARID1B ubiquitination. Oncotarget 2015-01-20 [PMID: 25596733] (WB)
• Nishibuchi G, Shibata Y, Hayakawa T et al. Physical and functional interactions between the histone H3K4 demethylase KDM5A and the nucleosome remodeling and deacetylase (NuRD) complex. J Biol Chem 2014-09-04 [PMID: 25190814] (IP, WB, Human)
• Yamamoto S, Wu Z, Russnes HG et al. JARID1B is a luminal lineage-driving oncogene in breast cancer. Cancer Cell 2014-06-16 [PMID: 24937458] (IP, IF/IHC, Human)
• Zou MR, Cao J, Liu Z et al. Histone Demethylase Jumonji AT-rich Interactive Domain 1B (JARID1B) Controls Mammary Gland Development by Regulating Key Developmental and Lineage Specification Genes. J Biol Chem 2014-06-20 [PMID: 24802759] (WB, Mouse)
• Klein BJ, Piao L, Xi Y et al. The histone-H3K4-specific demethylase KDM5B binds to its substrate and product through distinct PHD fingers. Cell Rep 2014-01-30 [PMID: 24412361]
• Wouters J, Stas M, Gremeaux L et al. The Human Melanoma Side Population Displays Molecular and Functional Characteristics of Enriched Chemoresistance and Tumorigenesis. PLoS One 2013-01-01 [PMID: 24098529]
• Hayami S, Yoshimatsu M, Veerakumarasivam A et al. Overexpression of the JmjC histone demethylase KDM5B in human carcinogenesis: involvement in the proliferation of cancer cells through the E2F/RB pathway. Mol Cancer. 2010-05-13 [PMID: 20226085]

Bioinformatics

• Gene Symbol: KDM5B