Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody

Images

 
Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352] - Analysis in control (vector only transfected HEK293T lysate) and kDM5B over-expression lysate (Co-expressed with a C-terminal ...read more
Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352] - Immunohistochemical staining of human rectum shows moderate to strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352] - Immunohistochemical staining of human skin shows moderate to strong nuclear positivity in epidermal cells.
Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352] - Immunohistochemical staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ...read more
Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352] - Immunohistochemical staining of human tonsil shows moderate to strong nuclear positivity in lymphoid cells.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:DGINSLERKLKRRLEREGLSSERWERVKKMRTPKKKKIKLSHPKDMNNFKLERERSYELVRSAETHSLPSDTSYSEQEDSE
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Rat (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KDM5B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:250 - 1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. IF fixation permeabilization: PFA/Triton X-100.
Control Peptide
Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Protein (NBP1-84352PEP)
Publications
Read Publications using
NBP1-84352 in the following applications:

  • IHC
    2 publications
  • IP
    2 publications
  • WB
    3 publications

Reactivity Notes

Expected species cross reactivity based on sequence homology: Mouse (86%). Reactivity reported in scientific literature (PMID: 24098529)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody

  • CT31
  • CT31FLJ10538
  • EC 1.14.11
  • EC 1.14.11.-
  • homolog 1A
  • Jarid1B
  • JARID1BFLJ16281
  • Jumonji, AT rich interactive domain 1B (RBP2-like)
  • jumonji, AT rich interactive domain 1B
  • KDM5B
  • Lysine (K)specific Demethylase 5B
  • Lysine (K)-specific Demethylase 5B
  • PLU-1
  • PLU-1FLJ12459
  • PLU1FLJ23670
  • PUT1
  • RBBP2H1
  • RBBP2H1AFLJ12491
  • RBP2-H1
  • Retinoblastoma-binding protein 2 homolog 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (NBP1-84352)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC, IP, WB.


Filter By Application
IHC
(2)
IP
(2)
WB
(3)
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Filter By Species
Human
(3)
Mouse
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using NBP1-84352 Applications Species
Wang L, Mao Y, Du G et al. Overexpression of JARID1B is associated with poor prognosis and chemotherapy resistance in epithelial ovarian cancer. Tumour Biol 2015 Apr [PMID: 25663457] (IHC, Human) IHC Human
Lu W, Liu S, Li B et al. SKP2 inactivation suppresses prostate tumorigenesis by mediating JARID1B ubiquitination. Oncotarget 2015 Jan 20 [PMID: 25596733] (WB) WB
Nishibuchi G, Shibata Y, Hayakawa T et al. Physical and Functional Interactions between the Histone H3K4 Demethylase KDM5A and the Nucleosome Remodeling and Deacetylase (NuRD) Complex. J Biol Chem 2014 Oct 17 [PMID: 25190814] (IP, WB, Human) IP, WB Human
Yamamoto S, Wu Z, Russnes HG et al. JARID1B is a luminal lineage-driving oncogene in breast cancer. Cancer Cell 2014 Jun 16 [PMID: 24937458] (IHC, IP, Human) IHC, IP Human
Zou MR, Cao J, Liu Z et al. Histone Demethylase Jumonji AT-rich Interactive Domain 1B (JARID1B) Controls Mammary Gland Development by Regulating Key Developmental and Lineage Specification Genes. J Biol Chem 2014 Jun 20 [PMID: 24802759] (WB, Mouse) WB Mouse
Klein BJ, Piao L, Xi Y et al. The histone-H3K4-specific demethylase KDM5B binds to its substrate and product through distinct PHD fingers. Cell Rep 2014 Jan 30 [PMID: 24412361]
Wouters J, Stas M, Gremeaux L et al. The Human Melanoma Side Population Displays Molecular and Functional Characteristics of Enriched Chemoresistance and Tumorigenesis. PLoS One 2013 [PMID: 24098529]
Hayami S, Yoshimatsu M, Veerakumarasivam A et al. Overexpression of the JmjC histone demethylase KDM5B in human carcinogenesis: involvement in the proliferation of cancer cells through the E2F/RB pathway. Mol Cancer 2010 Mar [PMID: 20226085]

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Product General Protocols

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol KDM5B
Entrez