Lyn Antibody (LYN-01) - BSA Free

Western Blot: Lyn Antibody (LYN-01) [NB500-519] - Fig. 1. Western Blotting analysis (non-reducing conditions) of Lyn using anti-Lyn (LYN-01). Lane 1: negative control Lane 2: RBL rat basophilic leukemia cell line Lane 3: JURKAT human peripheral blood T cell leukemia cell line Lane 4: A-431 human epidermoid carcinoma cell line Lane 5: U-937 human histiocytic lymphoma cell line.

Analysis of human LYN using mouse monoclonal antibody LYN-01 on lysates of Raji cell line and Jurkat cell line (LYN non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-LYN monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. LYN was detected around 55 kDa.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IP
• Clone: LYN-01
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Lyn Antibody (LYN-01) - BSA Free Summary

• Description: Novus Biologicals Mouse Lyn Antibody (LYN-01) - BSA Free (NB500-519) is a monoclonal antibody validated for use in WB, ICC/IF and IP. Anti-Lyn Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Bacterially expressed recombinant fragment of human Lyn, aa 8 - 238 (NM_001111097.2).
• Specificity: The LYN-01 reacts with Lyn (p56/p53), a non-receptor Src-family tyrosine kinase expressed in hematopoietic tissues.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LYN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:50
  • Western Blot 1-2 ug/ml
• Application Notes: For WB: Use non-reducing conditions. Purity is > 95% (by SDS-PAGE).

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Lyn Antibody (LYN-01) - BSA Free

• EC 2.7.10
• EC 2.7.10.2
• FLJ26625
• Hck-2
• JTK8
• Lyn
• tyrosine-protein kinase Lyn
• v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
• v-yes-1
• Yamaguchi sarcoma viral (v-yes-1) related oncogene homolog

Background

Lyn is a member of the src family of non-receptor protein tyrosine kinases that is predominantly expressed in haematopoietic tissues. Like all members of the src family, lyn is thought to participate in signal transduction from cell surface receptors that lack intrinsic tyrosine kinase activity. It is associated with a number of cell surface receptors including the B cell antigen receptor and immunoglobulin E receptor (FceRI).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lyn Antibody (NB500-519)(1)

Publication using NB500-519

• Hu H, Takano N, Xiang L et al. Hypoxia-inducible factors enhance glutamate signaling in cancer cells. Oncotarget 2014-10-15 [PMID: 25326682] (WB, Human)

Review for Lyn Antibody (NB500-519) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Hongxia Hu WB Human 09/12/2012

Summary

• Application: Western Blot
• Sample Tested: Hela whole cell lysate
• Species: Human

Bioinformatics

• Gene Symbol: LYN
• Entrez:
  • 4067(Human)
• Uniprot:
  • P07948()