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Lyn Antibody (LYN-01) - BSA Free

Images

 
Western Blot: Lyn Antibody (LYN-01) [NB500-519] - Fig. 1. Western Blotting analysis (non-reducing conditions) of Lyn using anti-Lyn (LYN-01). Lane 1: negative control Lane 2: RBL rat basophilic leukemia cell line Lane ...read more
Analysis of human LYN using mouse monoclonal antibody LYN-01 on lysates of Raji cell line and Jurkat cell line (LYN non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IP
Clone
LYN-01
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

Lyn Antibody (LYN-01) - BSA Free Summary

Immunogen
Bacterially expressed recombinant fragment of human Lyn, aa 8 - 238 (NM_001111097.2).
Specificity
The LYN-01 reacts with Lyn (p56/p53), a non-receptor Src-family tyrosine kinase expressed in hematopoietic tissues.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LYN
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:50
  • Western Blot 1-2 ug/ml
Application Notes
For WB: Use non-reducing conditions. Purity is > 95% (by SDS-PAGE).
Reviewed Applications
Read 1 Review rated 4
using
NB500-519 in the following applications:

Publications
Read Publication using
NB500-519 in the following applications:

  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Lyn Antibody (LYN-01) - BSA Free

  • EC 2.7.10
  • EC 2.7.10.2
  • FLJ26625
  • Hck-2
  • JTK8
  • Lyn
  • tyrosine-protein kinase Lyn
  • v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
  • v-yes-1
  • Yamaguchi sarcoma viral (v-yes-1) related oncogene homolog

Background

Lyn is a member of the src family of non-receptor protein tyrosine kinases that is predominantly expressed in haematopoietic tissues. Like all members of the src family, lyn is thought to participate in signal transduction from cell surface receptors that lack intrinsic tyrosine kinase activity. It is associated with a number of cell surface receptors including the B cell antigen receptor and immunoglobulin E receptor (FceRI).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lyn Antibody (NB500-519)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Human
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Review for Lyn Antibody (NB500-519) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB500-519:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot Lyn NB500-519
Enlarge
4
reviewed by:
Hongxia Hu
WB Human 09/12/2012
View

Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lyn Antibody (NB500-519) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on Lyn

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Recent Reviews

4
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4
1
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1
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Hongxia Hu
09/12/2012
Application: WB
Species: Human

Bioinformatics

Gene Symbol LYN
Entrez
Uniprot