Lyn Antibody (LYN-01)

Images

 
Western Blot: Lyn Antibody (LYN-01) [NB500-519] - Fig. 1. Western Blotting analysis (non-reducing conditions) of Lyn using anti-Lyn (LYN-01). Lane 1: negative control Lane 2: RBL rat basophilic leukemia cell line Lane ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IP
Clone
LYN-01
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Lyn Antibody (LYN-01) Summary

Immunogen
Bacterially expressed recombinant fragment of human Lyn, aa 8 - 238 (NM_001111097.2).
Specificity
The LYN-01 reacts with Lyn (p56/p53), a non-receptor Src-family tyrosine kinase expressed in hematopoietic tissues.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LYN
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:50
Application Notes
For WB: Use non-reducing conditions. Purity is > 95% (by SDS-PAGE).
Reviewed Applications
Read 1 Review rated 4
using
NB500-519 in the following applications:

Publications
Read Publication using
NB500-519 in the following applications:

  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Lyn Antibody (LYN-01)

  • EC 2.7.10
  • EC 2.7.10.2
  • FLJ26625
  • Hck-2
  • JTK8
  • Lyn
  • tyrosine-protein kinase Lyn
  • v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
  • v-yes-1
  • Yamaguchi sarcoma viral (v-yes-1) related oncogene homolog

Background

Lyn is a member of the src family of non-receptor protein tyrosine kinases that is predominantly expressed in haematopoietic tissues. Like all members of the src family, lyn is thought to participate in signal transduction from cell surface receptors that lack intrinsic tyrosine kinase activity. It is associated with a number of cell surface receptors including the B cell antigen receptor and immunoglobulin E receptor (FceRI).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lyn Antibody (NB500-519)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
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Filter By Species
Human
(1)
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Review for Lyn Antibody (NB500-519) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB500-519:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Lyn NB500-519
Enlarge
reviewed by:
Hongxia Hu
WB Human 09/12/2012
View

Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lyn Antibody (NB500-519) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB500-519AF405
Alexa Fluor 488 Labeled NB500-519AF488
Alexa Fluor 647 Labeled NB500-519AF647
Alexa Fluor 700 Labeled NB500-519AF700
DyLight 350 Labeled NB500-519UV
DyLight 405 Labeled NB500-519V
DyLight 488 Labeled NB500-519G
DyLight 680 Labeled NB500-519FR
DyLight 755 Labeled NB500-519IR

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Bioinformatics Tool for Lyn Antibody (NB500-519)

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Research Areas for Lyn Antibody (NB500-519)

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Blogs on Lyn

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Calculators

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Recent Reviews

4
5
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4
1
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0
2
0
1
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Hongxia Hu
09/12/2012
Application: WB
Species: Human

Bioinformatics

Gene Symbol LYN
Entrez
Uniprot