Lyn Antibody (10A6.2)

Western Blot: Lyn Antibody (10A6.2) [NBP2-29697] - A431 cell lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with ant-Lyn, clone 10A6.2 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescent detection system. Arrow indicates Lyn (~58 kDa).

Product Discontinued

Lyn Antibody (10A6.2) Summary

• Immunogen: KLH conjugated linear peptide corresponding to the C-terminus of Lyn.
• Specificity: Recognizes the C-terminus of Lyn (V-yes-1 Yamaguchi sarcoma viral related oncogene homolog).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: LYN
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot 1:500-1:1000
• Application Notes: Western Blot dilution of this lot detected Lyn in A431 cell lysate. Use in IHC reported in secitific publication PMID: 32768227
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse, Rat, Chicken. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Notes

Relevant Entrez Gene Numbers: NP_001104567

Alternate Names for Lyn Antibody (10A6.2)

• EC 2.7.10
• EC 2.7.10.2
• FLJ26625
• Hck-2
• JTK8
• Lyn
• tyrosine-protein kinase Lyn
• v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
• v-yes-1
• Yamaguchi sarcoma viral (v-yes-1) related oncogene homolog

Background

Lyn (V-yes-1 Yamaguchi sarcoma viral related oncogene homolog) is a member of the Src family of protein tyrosine kinases and is expressed primarily in hematopoietic cells and neural tissues. Lyn is involved in the regulation of cell activation by undergoing phosphorylation upon activation. Lyn activation triggers downstream signaling pathways that are mediated by Lyn phosphorylation of tyrosine residues of receptor proteins and recruitment and activation of other kinases including Syk, phospholipase CGamma 2 (PLCGamma 2) and phosphatidyl inositol-3 kinase. Lyn also plays an important role in B cells as a key regulator of both positive and negative signals, thus acting to maintain the balance between activation and inhibition. Deregulation of this balance can result in severe pathological consequences such as autoimmune disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lyn Antibody (NBP2-29697)(1)

Publication using NBP2-29697

• He S, Zheng G, Yang X et al. Avian leukosis virus subgroup J induces B cell anergy mediated by Lyn inhibited BCR signal transduction Veterinary Microbiology 2020-06-01 [PMID: 32768227] (WB, IF/IHC, Chicken)

Bioinformatics

• Gene Symbol: LYN
• Uniprot:
  • Q6NUK7(Human)