Ly49F Antibody (HBF-719) [Biotin]

• Reactivity: Mu
• Applications: Flow
• Clone: HBF-719
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin

Ly49F Antibody (HBF-719) [Biotin] Summary

• Immunogen: CHO-K1 cells transfected with B6 allele of Ly49F gene
• Specificity: B6 allele of the Ly49F receptor
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: Klra6
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Reactivity Notes

Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for Ly49F Antibody (HBF-719) [Biotin]

• Killer cell lectin like receptor 6
• Klra6
• Ly49F antigen
• Lymphocyte antigen 49F
• T cell surface glycoprotein Ly 49F

Background

The monoclonal antibody HBF-719 reacts with the B6 allele of Ly49F, a member of the lectin-like homodimeric Ly49 family of cell surface receptors.1 Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down-regulated. (2) These inhibitory receptors are also expressed on subpopulations of mouse CD8+ T cells. The HBF-719 antibody stains approx. 11% of NK1.1+CD3- splenocytes, and 3.5% (2.5 month old) to 10.0% (11 month old) of CD8+ spleen cells from C57BL/6 mice. (1)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Klra6