Ly49F Antibody (HBF-719) - Azide and BSA Free

Flow Cytometry: Ly49F Antibody (HBF-719) [NBP1-28096] - Analysis using the FITC conjugate of NBP1-28096. Multiple staining of C57BL/6 splenocytes.

Flow Cytometry: Ly49F Antibody (HBF-719) [NBP1-28096] - Analysis using the Biotin conjugate of NBP1-28096. Multiple staining of C57BL/6 splenocytes.

• Reactivity: Mu
• Applications: Flow
• Clone: HBF-719
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.5 mg/ml

Ly49F Antibody (HBF-719) - Azide and BSA Free Summary

• Immunogen: CHO-K1 cells transfected with B6 allele of Ly49F gene
• Specificity: B6 allele of the Ly49F receptor
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: Klra6
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry <= 1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.1M BBS (pH 8.2)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for Ly49F Antibody (HBF-719) - Azide and BSA Free

• Killer cell lectin like receptor 6
• Klra6
• Ly49F antigen
• Lymphocyte antigen 49F
• T cell surface glycoprotein Ly 49F

Background

The monoclonal antibody HBF-719 reacts with the B6 allele of Ly49F, a member of the lectin-like homodimeric Ly49 family of cell surface receptors.1 Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down-regulated. (2) These inhibitory receptors are also expressed on subpopulations of mouse CD8+ T cells. The HBF-719 antibody stains approx. 11% of NK1.1+CD3- splenocytes, and 3.5% (2.5 month old) to 10.0% (11 month old) of CD8+ spleen cells from C57BL/6 mice. (1)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ly49F Antibody (NBP1-28096)(4)

Publications using NBP1-28096

• Kim HJ, Nakagawa H, Choi JY et al. A narrow T cell receptor repertoire instructs thymic differentiation of MHC class Ib-restricted CD8+ regulatory T-cells The Journal of clinical investigation 2023-11-07 [PMID: 37934601] (Mouse)
• Strong BS, Ryken KO, Lee AE et al. Prenatal Allogeneic Tolerance in Mice Remains Stable Despite Potent Viral Immune Activation. J. Immunol. 2015-09-11 [PMID: 26363051] (FLOW, Mouse)
• Alhajjat AM, Strong BS, Lee AE et al. Prenatal Allospecific NK Cell Tolerance Hinges on Instructive Allorecognition through the Activating Receptor during Development J. Immunol. 2015-07-01 [PMID: 26136432] (FLOW, Mouse)
• Alhajjat A M, Strong B S et al. Trogocytosis as a mechanistic link between chimerism and prenatal tolerance. Chimerism 2013-10-15 [PMID: 24121538] (FLOW, Mouse)

Bioinformatics

• Gene Symbol: Klra6