Ly49F Antibody (HBF-719) [FITC]

Flow Cytometry: Ly49F Antibody (HBF-719) [FITC] [NBP1-28097] - Analysis of C57BL/6 splenocytes by multiple staining.

• Reactivity: Mu
• Applications: Flow
• Clone: HBF-719
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC

Ly49F Antibody (HBF-719) [FITC] Summary

• Immunogen: CHO-K1 cells transfected with B6 allele of Ly49F gene
• Specificity: B6 allele of the Ly49F receptor
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: Klra6
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for Ly49F Antibody (HBF-719) [FITC]

• Killer cell lectin like receptor 6
• Klra6
• Ly49F antigen
• Lymphocyte antigen 49F
• T cell surface glycoprotein Ly 49F

Background

The monoclonal antibody HBF-719 reacts with the B6 allele of Ly49F, a member of the lectin-like homodimeric Ly49 family of cell surface receptors.1 Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down-regulated. (2) These inhibitory receptors are also expressed on subpopulations of mouse CD8+ T cells. The HBF-719 antibody stains approx. 11% of NK1.1+CD3- splenocytes, and 3.5% (2.5 month old) to 10.0% (11 month old) of CD8+ spleen cells from C57BL/6 mice. (1)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Klra6