Ly-6A/E Antibody (RM0330-2M39) [Janelia Fluor® 549]

• Reactivity: Mu
• Applications: IHC
• Clone: RM0330-2M39
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Janelia Fluor 549

Ly-6A/E Antibody (RM0330-2M39) [Janelia Fluor® 549] Summary

• Immunogen: Mouse recombinant Ly-6A/E
• Isotype: IgG2
• Clonality: Monoclonal
• Host: Rat
• Gene: LY6A
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Ly-6A/E Antibody (RM0330-2M39) [Janelia Fluor® 549]

• Ly 6A/E
• Ly6A.2
• Ly6A/E
• Ly6E.1
• Lymphocyte antigen 6 complex locus A
• Lymphocyte antigen 6 complex locus E
• Retinoic acid induced gene E
• RIGE
• SCA1
• SCA-1
• SCA2
• Stem cell antigen 1
• Stem cell antigen 2
• T cell activating protein
• TAP
• Thymic shared antigen 1
• TSA1

Background

Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. (1-3) It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes. (4-6) In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes, whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Ly-6A/E+ lymphocytes. (1, 7-9) The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation. (1, 8, 9) Studies with MAb D7 suggest that Ly-6A/E may be involved in B and T lymphocyte responses, (9-12) and it appears that the antigen may be required for T-cell receptor-mediated T-cell activation. (3, 13)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LY6A