Lubricin/PRG4 Antibody

Product Discontinued

Lubricin/PRG4 Antibody Summary

• Description: Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.
• Immunogen: Peptide with sequence DYESFCAEVHNP corresponding to internal region according to NP_005798.2.
• Epitope: internal region
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PRG4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
• Application Notes: WB: Preliminary experiments in human bone marrow lysates gave no specific signal but low background.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Lubricin/PRG4 Antibody

• bG174L6.2 (MSF: megakaryocyte stimulating factor )
• bG174L6.2
• CACP
• FLJ32635
• HAPO
• Hemangiopoietin
• JCAP
• Lubricin
• megakaryocyte stimulating factor
• Megakaryocyte-stimulating factor
• MSF
• MSFproteoglycan 4, (megakaryocyte stimulating factor, articular superficial zoneprotein, camptodactyly, arthropathy, coxa vara, pericarditis syndrome)
• PRG4
• Proteoglycan 4
• Superficial zone proteoglycan
• SZP
• SZPHAPOarticular superficial zone protein

Background

The protein encoded by this gene is a large proteoglycan specifically synthesized by chondrocytes located at the surface of articular cartilage, and also by some synovial lining cells. This protein contains both chondroitin sulfate and keratan sulfate glycosaminoglycans. It functions as a boundary lubricant at the cartilage surface and contributes to the elastic absorption and energy dissipation of synovial fluid. Mutations in this gene result in camptodactyly-arthropathy-coxa vara-pericarditis syndrome. Multiple transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lubricin/PRG4 Antibody (NBP1-20940)(1)

Publication using NBP1-20940

• Jay GD, Torres JR, Warman ML et al. The role of lubricin in the mechanical behavior of synovial fluid. Proc Natl Acad Sci U S A 2007-04-10 [PMID: 17404241]

Bioinformatics

• Gene Symbol: PRG4
• Entrez:
  • 10216(Human)
• Uniprot:
  • NP_005798.2(Human)