LSP1 Antibody

Western Blot: LSP1 Antibody [24780002] - Samples: Lane 1, Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil, Target weight [kDa]: 50, 38, 37, 30 (splice variants) Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.

Immunohistochemistry: LSP1 Antibody [24780002] - Lymphoid tissues displayed moderate to strong cytoplasmic positivity. Normal epithelial and stromal cells were negative. Among malignant cells, most malignant lymphomas and a few cases of urothelial cancers expressed moderate cytoplasmic immunoreactivity. Image and statement courtesy of the Human Protein Atlas (HPA).

Immunohistochemistry: LSP1 Antibody [24780002] - staining for lymphocyte-specific protein 1 (LSP1) in human lymphoidal tissues. Lymphoid tissues of appendix displayed moderate to strong cytoplasmic positivity. Normal epithelial and stromal cells were negative. Most malignant lymphomas and a few cases of urothelial cancers expressed moderate cytoplasmic immunoreactivity (images not shown). These image are the courtesy of the Human Proteome Resource (HPR).

Immunohistochemistry: LSP1 Antibody [24780002] - Staining for lymphocyte-specific protein 1 (LSP1) in human lymphoidal tissues. Lymphoid tissues of lymph nodes displayed moderate to strong cytoplasmic positivity. Normal epithelial and stromal cells were negative. Most malignant lymphomas and a few cases of urothelial cancers expressed moderate cytoplasmic immunoreactivity (images not shown). These image are the courtesy of the Human Proteome Resource (HPR).

Immunohistochemistry: LSP1 Antibody [24780002] - Staining for lymphocyte-specific protein 1 (LSP1) in human lymphoidal tissues. Lymphoid tissues of tonsil displayed moderate to strong cytoplasmic positivity. Normal epithelial and stromal cells were negative. Most malignant lymphomas and a few cases of urothelial cancers expressed moderate cytoplasmic immunoreactivity (images not shown). These image are the courtesy of the Human Proteome Resource (HPR).

ELISA: LSP1 Antibody [24780002]

Product Discontinued

LSP1 Antibody Summary

• Immunogen: This antibody is specific for the C Terminus Region of the target protein.
• Specificity: This product is specific for Human LSP1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LSP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:5000-1:20000
• Application Notes: This antibody is useful in ELISA, Immunohistochemistry-Paraffin and Western Blot.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.

Alternate Names for LSP1 Antibody

• 47 kDa actin binding protein
• 47 kDa actin-binding protein
• 52 kDa phosphoprotein
• F-actin binding and cytoskeleton associated protein
• leufactin (leukocyte F-actin binding protein)
• leukocyte-specific protein 1
• lymphocyte-specific protein 1
• lymphocyte-specific protein pp52
• pp52
• WP34Lymphocyte-specific antigen WP34

Background

This gene encodes an intracellular F-actin binding protein. The protein is expressed in lymphocytes, neutrophils, macrophages, and endothelium and may regulate neutrophil motility, adhesion to fibrinogen matrix proteins, and transendothelial migration. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LSP1 Antibody (24780002)(2)

Publications using 24780002

• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.

Bioinformatics

• Gene Symbol: LSP1
• Entrez:
  • 4046(Human)
• Uniprot:
  • P33241(Human)