LRTM1 Antibody (2381D) [Unconjugated]

Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human LRTM1 and eGFP. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human LRTM1 Monoclonal Antibody (Catalog # MAB10046) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LRTM1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

HEK293 human embryonic kidney cell line transfected with (A) human LRTM1 or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Human LRTM1 Monoclonal Antibody (Catalog # MAB10046) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.

LRTM1 was detected in immersion fixed TK-6 human lymphoblastoid cell line (left panel; positive stain) and A549 human lung carcinoma cell line (right panel; negative stain) using Rabbit Anti-Human LRTM1 Monoclonal Antibody (Catalog # MAB10046) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

LRTM1 was detected in immersion fixed paraffin-embedded sections of human spleen using Rabbit Anti-Human LRTM1 Monoclonal Antibody (Catalog # MAB10046) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, Flow, IHC, CyTOF-ready, ICC/IF
• Clone: 2381D
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

LRTM1 Antibody (2381D) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide containing human LRTM1
  Accession # Q9HBL6
• Specificity: Detects human LRTM1 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 3-25 ug/mL
  • Immunohistochemistry 3-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LRTM1 Antibody (2381D) [Unconjugated]

• HT017
• leucine-rich repeat and transmembrane domain-containing protein 1
• leucine-rich repeats and transmembrane domains 1
• LRTM1

Background

LRTM1 belongs to the extracellular LRRs superfamily.  LRTM1 is involved in positive regulation of synapse assembly and is an integral component of the membrane.  LRTM1 has the highest expression level in pineal body.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9HBL6()