LRIG2 Antibody (990332) [Unconjugated]

LRIG2 was detected in immersion fixed WM-115 human malignant melanoma cell line using Mouse Anti-Human LRIG2 Monoclonal Antibody (Catalog # MAB19411) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

LRIG2 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human LRIG2 Monoclonal Antibody (Catalog # MAB19411) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

LRIG2 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human LRIG2 Monoclonal Antibody (Catalog # MAB19411) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC, ICC/IF
• Clone: 990332
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

LRIG2 Antibody (990332) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human LRIG-2
  Gly41-Thr805
  Accession # O94898
• Specificity: Detects human LRIG2 in immunocytochemistry and immunohistochemistry.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-25 ug/mL
  • Immunohistochemistry 1.7-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LRIG2 Antibody (990332) [Unconjugated]

• KIAA0806
• leucine-rich repeats and immunoglobulin-like domains 2
• leucine-rich repeats and immunoglobulin-like domains protein 2
• LIG2
• LRIG2
• UFS2

Background

LRIG2 (Leucine-rich Repeats and Ig-like domains-2; also LIG-2) is an approximately 119-132 kDa glycoprotein that belongs to the LRIG gene family. The LRIG2 protein was predicted to have the same domain organization as LRIG1with a signal peptide, an extracellular part containing 15 leucine-rich repeats and three immunoglobulin-like domains, a transmembrane domain, and a cytoplasmic tail. Over aa 41‑805, human LRIG2 shares 94%, 93% and 92% aa sequence identity with mouse, rat and macaque LRIG2 respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O94898()