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LRCH1 Antibody

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Western Blot: LRCH1 Antibody [NBP2-44282] - Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. NBP2-44282 was used for IP at 6 ug per reaction. LRCH1 was also immunoprecipitated by NBP2-44281
Independent Antibodies: Western Blot: LRCH1 Antibody [NBP2-44282] - Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using ...read more
Biological Strategies: Western Blot: LRCH1 Antibody [NBP2-44282] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

  

Biological Strategies

      

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LRCH1 Antibody Summary

Immunogen
The epitope recognized by this antibody maps to a region between residue 375 to 425 of human Leucine-rich repeat and calponin homology domain-containing protein 1 using the numbering given in entry Q9Y2L9.3 (GeneID 23143).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LRCH1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:2000 - 1:10000

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LRCH1 Antibody

  • CHDC1
  • FLJ41660
  • KIAA1016
  • leucine-rich repeat and calponin homology domain-containing protein 1
  • leucine-rich repeats and calponin homology (CH) domain containing 1
  • neuronal protein 81
  • NP81

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: Asporin Antibody [NBP2-15492] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
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Immunohistochemistry-Paraffin: OXTR Antibody [NBP2-76347] - Negative Control showing staining of paraffin embedded Human Ovary, with no primary antibody.
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Western Blot: N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody [NBP1-32899] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract7.5% SDS-PAGE GALNS antibody dilution: 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
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Osteopontin/OPN was detected in perfusion fixed frozen sections of mouse thymus using Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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Western Blot: RhoB Antibody [NBP2-20154] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RhoB antibody diluted at 1:1000.
Western Blot: RhoB Antibody [NBP2-20154] - Sample (30 ug of whole cell lysate) A: U87-MG 12% SDS PAGE gel, diluted at 1:500.
Western Blot: RhoB Antibody [NBP2-20154] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.
Species: Hu, Mu, Rt
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Bioinformatics

Gene Symbol LRCH1