Recombinant Human LPCAT1 His Protein

SDS-Page: LPCAT1 Protein [NBP2-23206]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.25 mg/ml

Recombinant Human LPCAT1 His Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 79-534 of Human LPCAT1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSSAEKEPE QPPALWRKVV DFLLKAIMRT MWFAGGFHRV AVKGRQALPT EAAILTLAPH SSYFDAIPVT MTMSSIVMKA ESRDIPIWGT LIQYIRPVFV SRSDQDSRRK TVEEIKRRAQ SNGKWPQIMI FPEGTCTNRT CLITFKPGAF IPGAPVQPVV LRYPNKLDTI TWTWQGPGAL EILWLTLCQF HNQVEIEFLP VYSPSEEEKR NPALYASNVR RVMAEALGVS VTDYTFEDCQ LALAEGQLRL PADTCLLEFA RLVRGLGLKP EKLEKDLDRY SERARMKGGE KIGIAEFAAS LEVPVSDLLE DMFSLFDESG SGEVDLRECV VALSVVCRPA RTLDTIQLAF KMYGAQEDGS VGEGDLSCIL KTALGVAELT VTDLFRAIDQ EEKGKITFAD FHRFAEMYPA FAEEYLYPDQ THFESCAETS PAPIPNGFCA DFSPENSDAG RKPVRKKLD

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: LPCAT1
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 53.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 1 M UREA, 10% glycerol
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human LPCAT1 His Protein

• 1-acylglycerophosphocholine O-acyltransferase
• Acetyl-CoA:lyso-PAF acetyltransferase
• Acetyl-CoA:lyso-platelet-activating factor acetyltransferase
• acyl-CoA:lysophosphatidylcholine acyltransferase 1
• acyltransferase like 2
• Acyltransferase-like 2
• AYTL2
• EC 2.3.1.-
• EC 2.3.1.23,1-alkylglycerophosphocholine O-acetyltransferase
• EC 2.3.1.67
• FLJ12443
• FLJ41609
• LPC acyltransferase 1
• lpcat
• LPCAT-1
• Lyso-PAF acetyltransferase
• lysoPAFAT
• LysoPC acyltransferase 1
• lysophosphatidylcholine acyltransferase 1
• PFAAP3
• Phosphonoformate immuno-associated protein 3
• regulated by phosphonoformate

Background

Lysophosphatidylcholine acyltransferase 1, also known as LPCAT1, belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. It is a key enzyme for remodeling phospholipids, including phosphatidylcholine. LPCAT1 possesses both acyltransferase and acetyltransferase activities. It mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC). Recombinant human LPCAT1 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: LPCAT1