LMO7 Antibody

Western Blot: LMO7 Antibody [NB100-1360] - Staining of human lung lysate (RIPA buffer, 30 ug total protein). Antibody at 1 ug/mL. Detected by chemiluminescence.

Immunohistochemistry-Frozen: LMO7 Antibody [NB100-1360] - LMO7 antibody stained hair cells in cochlea cross-sections of mouse CD1 P1. Alexa Fluor 488 used as secondary antibody to LMO7 for visualization. Image submitted by a verified customer review.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

LMO7 Antibody Summary

• Description: Novus Biologicals Goat LMO7 Antibody (NB100-1360) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-LMO7 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence ESTCVSASLPRS corresponding to internal region according to AAD33924.1.
• Specificity: Please note that the amino acid sequence of the immuning peptide differs one amino acid from the current NCBI accession numbers (NP_005349.3, NP_056667.2). This antibody is expected to recognise both reported isoforms (NP_005349.3; NP_056667.2).
• Predicted Species: Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LMO7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5 - 2 ug/mL
• Application Notes: WB: Approx. 70-75 kDa band and approx. 30 kDa band observed in human lung lysates (predicted MW of 92 kDa band according to AAD33924.1) and is in aggrement with scientific literature (Putilina et al). A minor band of unknown identity was also consistently observed at approx. 50 kDa. LMO7 antibody validated for IHC-Frozen from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LMO7 Antibody

• FBX20F-box only protein 20
• KIAA0858FBXO20F-box protein Fbx20
• LIM domain 7
• LIM domain only 7
• LIM domain only protein 7
• LMO-7
• LOMP
• zinc-finger domain-containing protein

Background

This protein contains a calponin homology (CH) domain, a PDZ domain, and a LIM domain. An F-box (FBX) domain is present in alternative splice variants. Members of the LIM protein family carry the LIM domain, a unique cysteine-rich zinc-binding domain. Members of the FBX protein family are involved in protein-protein interactions. The encoded protein may be involved in protein-protein interactions. Multiple alternative splice variants have been described but their full-length sequences have not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LMO7 Antibody (NB100-1360)(1)

Publication using NB100-1360

• Putilina T, Jaworski C, Gentleman S et al. Analysis of a human cDNA containing a tissue-specific alternatively spliced LIM domain. Biochem Biophys Res Commun 1998-11-18 [PMID: 9826547]

Review for LMO7 Antibody (NB100-1360) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: CD1 Mouse .

reviewed by: Gianluca Sampieri IHC-Fr CD1 Mouse 12/18/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Cochlea Cross Section Postnatal Day,Cochlea Cross-section P1
• Species: CD1 Mouse
• Lot: Lot P1 (NB100-1360)

Bioinformatics

• Gene Symbol: LMO7
• Entrez:
  • 4008(Human)
• Uniprot:
  • AAD33924.(Human)