LIX Antibody (135803) [Alexa Fluor® 532]

• Reactivity: Rt
• Applications: WB, B/N, ELISA(Cap), ELISA(Det)
• Clone: 135803
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

LIX Antibody (135803) [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant rat LIX
  Ala38-Arg125, Ala38-Lys117
  Accession # P97885.1
• Specificity: Detects rat LIX in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) LIX is observed, 5% cross-reactivity with recombinant rat (rr) CXCL2 is observed, and no cross-reactiv
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • Neutralization
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LIX Antibody (135803) [Alexa Fluor® 532]

• LIX

Background

LIX (Liposaccharide-Induced CXC chemokine; also GARG-8) is a secreted 8-10 kDa member of the Intercrine alpha (or CxC) family of chemokines.  It is widely expressed, being produced by diverse cell types such as fibroblasts, thymic epithelium, platelets, vascular endothelium, hepatocytes, lung type II alveolar cells and ileal columnar epithelium.  As a chemokine, LIX demonstrates chemotactic properties.  It induces the chemotaxis of neutrophils and endothelial cells, and also promotes TNF-alpha secretion from mast cells and macrophages.  Notably, circulating LIX is not derived from fibroblasts, but platelets.  This suggests that neutrophil homeostasis/chemotaxis is a function of local resident cell activation and LIX secretion, not generally circulating LIX.  Rat LIX is synthesized as a 130 amino acid (aa) precursor that contains a 37 aa signal sequence plus a 93 aa mature region (aa 38-130) that likely possesses a cleavable 13 aa C-terminus (SwissProt #:P97885).  The mature region possesses a receptor-binding ELR/GluLeuArg motif between aa 47-50, and an alpha -family characteristic CxC motif between aa 50-52.  Although there are no known splice variants of rat LIX, based on mouse, considerable proteolytic processing likely occurs at both the N- and C-termini over aa 38-130.  This may reduce the MW in SDS-Page by as much as 3 kDa.  The majority of LIX could be expected to start between aa 43-46, and this is likely to be positively correlated with bioactivity.  Over aa 38-117, rat LIX shares 73% aa sequence identity with mouse LIX.  Although not a strict ortholog, rat LIX also shares 64% aa sequence identity with human GCP-2.

 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.