Lipin 2 Antibody

Product Discontinued

Lipin 2 Antibody Summary

• Immunogen: Peptide with sequence CYWRDPIPEVDLDDLS corresponding to C-Terminus according to NP_055461.1.
• Epitope: CYWRDPIPEVDLDDLS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LPIN2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
• Application Notes: WB: Preliminary experiments gave an approx. 28 kDa band in human adipose tissue and human skeletal muscle lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 99.4 kDa band according to NP_055461. The 28 kDa band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for Lipin 2 Antibody

• EC 3.1.3.4
• KIAA0249lipin-2
• Lipin 2
• Lipin-2
• LPIN2
• phosphatidate phosphatase LPIN2

Background

Lipin 2 plays important roles in controlling the metabolism of fatty acids at differents levels. Lipin 2 acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Lipin 2 also acts also as a nuclear transcriptional coactivator for PPARGC1A to modulate lipid metabolism By similarity. Defects in LPIN2 are the cause of Majeed syndrome (MAJEEDS) [MIM:609628]. An autosomal recessive syndrome characterized by chronic recurrent multifocal osteomyelitis that is of early onset with a lifelong course, congenital dyserythropoietic anemia that presents as hypochromic, microcytic anemia during the first year of life and ranges from mild to transfusion-dependent, and transient inflammatory dermatosis, often manifesting as Sweet syndrome (neutrophilic skin infiltration).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lipin 2 Antibody (NBP1-52125)(1)

Publication using NBP1-52125

• Phan J, Peterfy M, Reue K. Lipin expression preceding peroxisome proliferator-activated receptor-gamma is critical for adipogenesis in vivo and in vitro. J Biol Chem 2004-07-09 [PMID: 15123608]

Bioinformatics

• Gene Symbol: LPIN2
• Entrez:
  • 9663(Human)
• Uniprot:
  • NP_055461.1(Human)