LIN7 Antibody

Western Blot: LIN7 Antibody [NBP2-99481] - Lane A: Mouse brain tissue lysateLane B: Rat brain tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:26 kDa

Immunocytochemistry/Immunofluorescence: LIN7 Antibody [NBP2-99481] - Immunofluorescence staining of LIN7 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human LIN7 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and cell membrane.

Immunoprecipitation: LIN7 Antibody [NBP2-99481] - Lane A:0.5 mg HepG2 Whole Cell Lysate4 uL anti-ATP6V1C1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ATP6V1C1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 44 kDaObserved band size :45 kDa

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

LIN7 Antibody Summary

• Immunogen: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human LIN7.
• Predicted Species: Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LIN7A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:300-1:10000
  • Immunoprecipitation 5-10 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Notes

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Alternate Names for LIN7 Antibody

• hLin-7
• lin-7 homolog A (C. elegans)
• LIN7
• LIN-7A
• MALS1
• MALS-1MGC148143
• Mammalian lin-seven protein 1
• Tax interaction protein 33
• TIP-33mammalian LIN-7 1
• veli-1
• VELI1protein lin-7 homolog A
• vertebrate LIN7 homolog 1
• Vertebrate lin-7 homolog 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LIN7A