LIN-41/TRIM71 Antibody [Unconjugated]

Western blot shows lysates of BG0IV human embryonic stem cells and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human LIN-41 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5104) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LIN-41 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

LIN-41 was detected in immersion fixed BG01V human embryonic stem cells using Human LIN-41 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5104) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western lane view shows lysates of BG01V human embryonic stem cells, loaded at 0.2 mg/mL. A specific band was detected for LIN‑41 at approximately 97 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human LIN‑41 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5104) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

LIN-41/TRIM71 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human LIN-41
  Val386-Phe868
  Accession # Q2Q1W2
• Specificity: Detects human LIN-41 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: TRIM71
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LIN-41/TRIM71 Antibody [Unconjugated]

• LIN41
• LIN-41
• TRIM71
• tripartite motif containing 71
• tripartite motif-containing protein 71

Background

LIN-41 (lineage-41; also TRIM/tripartite motif-containing protein 71) is a 94 kDa (predicted) cytosolic member of the RBCC (RING finger, B-Box Coiled-Coil) family of proteins. It is expressed in developing limbs, DRG and branchial arches and apparently regulates the timed expression of proliferation and differentiation-associated genes. Human LIN-41 is 868 amino acids (aa) in length. It contains a RING-type zinc finger region (aa 12-95), a His-rich segment (aa 146‑157), two B Box zinc fingers that bind DNA (aa 194-314), a coiled-coil motif that binds protein (aa 391-427), one filamin repeat and six NHL repeats that regulate gene translation (aa 593‑868). Over aa 386-868, human LIN-41 is 97%, 95%, and 94% aa identical to canine, mouse, and chicken LIN-41, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LIN-41/TRIM71 Antibody (AF5104)(4)

Publications using AF5104

• Chang, JJ;Lin, T;Jhang, XY;Chan, SP; hnRNP Q/SYNCRIP interacts with LIN28B and modulates the LIN28B/let-7 axis in human hepatoma cells PloS one 2024-07-08 [PMID: 38976670] (Western Blot, Human)
• Qiuying Liu, Xiaoli Chen, Mariah K Novak, Shaojie Zhang, Wenqian Hu Repressing Ago2 mRNA translation by Trim71 maintains pluripotency through inhibiting let-7 microRNAs eLife 2/18/2021 [PMID: 33599613]
• Qiuying Liu, Xiaoli Chen, Mariah K Novak, Shaojie Zhang, Wenqian Hu Repressing Ago2 mRNA translation by Trim71 maintains pluripotency through inhibiting let-7 microRNAs eLife 2021-02-18 [PMID: 33599613] (Western Blot, Mouse)
• Bronevetsky , Yelena, Villarino , Alejandr, Eisley , Christop, Barbeau , Rebecca, Barczak , Andrea J, Heinz , Gitta A, Kremmer , Elisabet, Heissmeyer , Vigo, McManus , Michael, Erle , David J, Rao , Anjana, Ansel , K Mark T cell activation induces proteasomal degradation of Argonaute and rapid remodeling of the microRNA repertoire. J Exp Med, 2013-02-04;210(2):417-32. 2013-02-04 [PMID: 23382546] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: TRIM71
• Uniprot:
  • Q2Q1W2()