LH beta Antibody

Images

 
Western blot shows lysates of human pituitary gland tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human LH beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8016) followed by ...read more
LH beta was detected in immersion fixed paraffin-embedded sections of human pituitary adenoma using Sheep Anti-Human LH beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8016) at 1 µg/mL overnight at4 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

LH beta Antibody Summary

Immunogen
E. coli-derived recombinant human LH beta
Ser21-Leu141
Accession # P01229
Specificity
Detects human LH beta in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
LHB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Control
LH beta Lysate (NBP2-66224)

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LH beta Antibody

  • CGB4
  • hLHB
  • interstitial cell stimulating hormone, beta chain
  • LH beta
  • LHB
  • LSH-B
  • LSH-beta
  • luteinizing hormone beta polypeptide
  • luteinizing hormone beta subunit
  • lutropin beta chain
  • Lutropin beta
  • lutropin subunit beta

Background

LH beta (Luteinizing Hormone subunit beta; also LSH-B and lutropin beta chain) is a 17-19 kDa member of the glycoprotein hormone subunit beta family, cysteine knot superfamily of proteins. It is synthesized by gonadotrophs in the anterior pituitary, and noncovalently heterodimerizes with the 21-23 kDa common glycoprotein alpha chain, forming 40-42 kDa LH. LH has effects on at least three organ systems. In the ovary, LH induces the synthesis of testosterone, which is quickly converted to estradiol. Estradiol subsequently induces a bolus release of LH from the pituitary, resulting in ovulation. LH now drives the formation of the corpus luteum (or yellow body, from luteus meaning yellow) with the production of progesterone. In the testis, LH induces Leydig cell production of testosterone that activates genes in Sertoli cells drives sperm formation. And in the adrenal, LH appears to induce secretion of sulfated DHEA, a precursor to androgens. Mature human LH beta is 121 amino acids (aa) in length (aa 21-141). It contains three intrachain disulfide bonds that form a cysteine knot, plus one utilized N-linked glycosylation site at Asn50 that incorporates both fucose and sulfate on galactose. The presence of carbohydrate is necessary for bioactivity. Multiple isoforms of LH exist, and likely represent variations in carbohydrate structure. Full-length human LH beta shares 82% and 72% aa sequence identity between human chorionic gonadotrophin/CG beta and mouse LH beta subunits, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LHB
Entrez
Uniprot