LH beta Antibody [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 405

LH beta Antibody [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human LHβ
  Ser21-Leu141
  Accession # P01229
• Specificity: Detects human LH beta in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LH beta Antibody [Alexa Fluor® 405]

• CGB4
• hLHB
• interstitial cell stimulating hormone, beta chain
• LH beta
• LHB
• LSH-B
• LSH-beta
• luteinizing hormone beta polypeptide
• luteinizing hormone beta subunit
• lutropin beta chain
• Lutropin beta
• lutropin subunit beta

Background

LH beta (Luteinizing Hormone subunit beta; also LSH-B and lutropin beta chain) is a 17-19 kDa member of the glycoprotein hormone subunit beta family, cysteine knot superfamily of proteins. It is synthesized by gonadotrophs in the anterior pituitary, and noncovalently heterodimerizes with the 21-23 kDa common glycoprotein alpha chain, forming 40-42 kDa LH. LH has effects on at least three organ systems. In the ovary, LH induces the synthesis of testosterone, which is quickly converted to estradiol. Estradiol subsequently induces a bolus release of LH from the pituitary, resulting in ovulation. LH now drives the formation of the corpus luteum (or yellow body, from luteus meaning yellow) with the production of progesterone. In the testis, LH induces Leydig cell production of testosterone that activates genes in Sertoli cells drives sperm formation. And in the adrenal, LH appears to induce secretion of sulfated DHEA, a precursor to androgens. Mature human LH beta is 121 amino acids (aa) in length (aa 21-141). It contains three intrachain disulfide bonds that form a cysteine knot, plus one utilized N-linked glycosylation site at Asn50 that incorporates both fucose and sulfate on galactose. The presence of carbohydrate is necessary for bioactivity. Multiple isoforms of LH exist, and likely represent variations in carbohydrate structure. Full-length human LH beta shares 82% and 72% aa sequence identity between human chorionic gonadotrophin/CG beta and mouse LH beta subunits, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.