Legumain/Asparaginyl Endopeptidase Overexpression Lysate Summary
Legumain/Asparaginyl Endopeptidase Transient Overexpression Lysate
Expression Host: HEK293T
Protein Tag: C-MYC/DDK
You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
This product is intended for use as a positive control in Western Blot.
Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Lysate Details for Array
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for Legumain/Asparaginyl Endopeptidase Overexpression Lysate
- Asparaginyl Endopeptidase
- cysteine protease 1
- Protease, cysteine 1
- protease, cysteine, 1 (legumain)
- PRSC1EC 22.214.171.124
This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq]. Transcript Variant: This variant (2) has an additional exon in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
Customers Who Viewed This Item Also Viewed...
Species: Ch, Hu
Applications: IHC, IHC-P, IP, KD, WB
Applications: ICC/IF, IHC, IHC-P, WB
Applications: IHC, WB
Species: Ca, Hu, Pm, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Applications: ICC, IHC, IP, KO, Simple Western, WB
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, IP, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
Applications: IHC, IHC-P, IP, WB (-)
Applications: IHC, IHC-P
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Applications: IHC, IP, Simple Western, WB
Applications: IHC, IHC-P, WB
Applications: ELISA, AP, PA, WB
Applications: CyTOF-ready, Flow, IP, WB
Applications: IHC, WB
Species: Hu, Mu, Md, Pm, Rt
Applications: ChIP, EM, Flow-IC, Flow, ICC/IF, IP, In vitro, WB
Applications: B/N, CyTOF-ready, EM, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Publications for Legumain/Asparaginyl Endopeptidase Lysate (NBL1-12508) (0)
There are no publications for Legumain/Asparaginyl Endopeptidase Lysate (NBL1-12508).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for Legumain/Asparaginyl Endopeptidase Lysate (NBL1-12508) (0)
There are no reviews for Legumain/Asparaginyl Endopeptidase Lysate (NBL1-12508).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for Legumain/Asparaginyl Endopeptidase Lysate (NBL1-12508) (0)
Additional Legumain/Asparaginyl Endopeptidase Products
Bioinformatics Tool for Legumain/Asparaginyl Endopeptidase Lysate (NBL1-12508)
Discover related pathways, diseases and genes to Legumain/Asparaginyl Endopeptidase Lysate (NBL1-12508). Need help?
Read the Bioinformatics Tool Guide
for instructions on using this tool.
Research Areas for Legumain/Asparaginyl Endopeptidase Lysate (NBL1-12508)
Find related products by research area.
Blogs on Legumain/Asparaginyl Endopeptidase