Lefty-2 Antibody (836101) [Alexa Fluor® 750]

• Reactivity: Mu
• Applications: ICC/IF
• Clone: 836101
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 750

Lefty-2 Antibody (836101) [Alexa Fluor® 750] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Lefty-2
  Phe78-Leu368
  Accession # P57785
• Specificity: Detects mouse Lefty-2 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Lefty-A or recombinant mouse Lefty-1 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lefty-2 Antibody (836101) [Alexa Fluor® 750]

• Ebaf
• Endometrial Bleeding-Associated Factor
• LEFTA
• Left-Right Determination Factor 2
• Left-right determination factor A
• Lefty-2
• LEFTYA
• Protein Lefty-2
• Protein Lefty-A
• TGFB4
• TGF-beta 4
• TGF-beta-4
• Transforming Growth Factor Beta-4

Background

Lefty-2 (Left-right determination factor 2; also Lefty-B [in human]) is a atypical member of the TGF-beta family of proteins. It is expressed during early embryogenesis in both the primitive streak and left-side lateral plate mesoderm. In the adult, Lefty-2 appears in oviduct epithelium. Lefty-2 acts in a manner reminiscent of that for Chordin and Noggin, and it is assumed that Lefty-2 is an antagonist of BMP activity. Notably, Lefty-2 and Nodal are likely under the control of Lefty-1, and thus all three molecules would appear to contribute to the creation of a left side-type body plan. Mouse Lefty-2 is synthesized as a 368 amino acid (aa) preproprecursor. It contains a 21 aa signal sequence, plus a 347 aa, 41-42 kDa bioactive proprecursor that may undergo proteolytic processing at one of two downstream cleavage sites. If cleavage occurs after Arg77, the resulting 33-34 kDa mature form (aa 78-368) is biologically inactive; if cleavage occurs after Arg135, the resulting 27-28 kDa mature form (aa 136-368) is biologically active. Lefty-2 is not a covalent homodimer and has been suggested to act as a monomer. Over aa 78-368, mouse Lefty-2 shares 94%, 83% and 95% aa sequence identity with rat Lefty-2, human Lefty-B and mouse Lefty-1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.