Lefty-1 Antibody (146903) [Alexa Fluor® 488]

• Reactivity: Mu
• Applications: WB
• Clone: 146903
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 488

Lefty-1 Antibody (146903) [Alexa Fluor® 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Lefty-1
  Leu136-Pro368
  Accession # Q64280.1
• Specificity: Detects mouse Lefty-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows 10-25% cross-reactivity with recombinant human (rh) Lefty-A and no cross-reactivity with rmArtemin, rhCripto, rdDpp, rhGDNF, rrGDNF,
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lefty-1 Antibody (146903) [Alexa Fluor® 488]

• AI450052
• EBAF
• Leftb
• lefty
• Lefty1
• Lefty-1
• Lefty-B
• Stra3
• TGFB4

Background

Lefty was first identified in a screen for undifferentiated cell-specific cDNAs from the P19 mouse embryonal carcinoma cells. Its mRNA expression on the left side of the developing embryo earned it the name “lefty”. Two genes exist in mouse (Lefty-1 and Lefty-2) and two in humans (Lefty-A (ebaf) and Lefty-B). By amino acid sequence, mouse Lefty‑1 and -2 are more similar to each other (90%) than to either Lefty-A or -B in humans (81-82% identical). Lefty contains the six cysteine residues that are conserved among TGF-beta related proteins and that are necessary to form the cysteine-knot structure. However, lefty is distinct from other family members in that it has two RXXR cleavage sites, a longer carboxy terminal sequence, and it lacks the cysteine residue required for intermolecular disulfide linkage. Thus, mature forms of lefty are larger than mature forms of other TGF-beta -related proteins. Mouse Lefty-1 is differentially processed depending on the cells in which it is synthesized, and both processing sites can be utilized. Lefty homologues have been identified in other vertebrate organisms including chick, frog, and zebrafish. Although the amino acid sequence identity is not well conserved among vertebrate species, the expression pattern of lefty on the left side is well conserved. Lefty-1 is expressed strongly on the left side of the prospective floor plate and weakly in the left half of the lateral plate mesoderm in E8 mice embryos. In all species examined, lefty proteins function in patterning left-right asymmetry of the developing organ systems such as the heart and lung. Lefty acts as an antagonist to Nodal signaling, potentially by competing for binding to a common receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.