LDB3 Antibody

Western Blot: LDB3 Antibody [NB100-2445] - Staining of Human Skeletal Muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: LDB3 Antibody [NB100-2445] - Negative Control showing staining of paraffin embedded Human Heart, with no primary antibody.

Immunohistochemistry-Paraffin: LDB3 Antibody [NB100-2445] - (6ug/ml) staining of paraffin embedded Human Heart. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Hu, Mu, Rt, Po, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

LDB3 Antibody Summary

• Immunogen: Peptide with sequence C-KSQNKPEDEADE corresponding to internal region according to NP_009009.1, NP_001073583.1, NP_001073584.1, NP_001073585.1.
• Epitope: C-KSQNKPEDEADE
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LDB3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 6-7 ug/ml
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 0.1 - 1 ug/ml
• Application Notes: WB: WB experiments gave bands at approx. 90 kDa band and 35 kDa band in human skeletal muscle lysate after 1 ug/ml antibody staining. These bands correspond to earlier findings are reported in scientific literature with different antibodies (PMID: 12499364). This protein has a calculated MW of 77.1 kDa band according to NP_009009.1.
• Control:

  Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LDB3 Antibody

• CMD1C
• CYPHER
• KIAA01613
• KIAA0613FLJ35865
• LDB3Z1
• LDB3Z4
• LIM domain binding 3
• LIM domain-binding protein 3
• ORACLE
• PDLIM6
• PDZ and LIM domain 6
• Protein cypher
• ZASPLVNC3
• Z-band alternatively spliced PDZ-motif protein

Background

Gene ID(s): 11155 (human)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LDB3 Antibody (NB100-2445)(4)

Publications using NB100-2445

• Leung MC, Hitchen PG, Ward DG et al. Z-band alternatively spliced PDZ-motif protein (ZASP) is the major O-linked-beta-N-acetylglucosamine-substituted protein in human heart myofibrils J Biol Chem 2012-12-27 [PMID: 23271734] (WB, Human)
• Arimura T, Inagaki N, Hayashi T et al. Impaired binding of ZASP/Cypher with phosphoglucomutase 1 is associated with dilated cardiomyopathy. Cardiovasc Res. 2009-05-13 [PMID: 19377068]
• Selcen D, Engel AG. Mutations in ZASP define a novel form of muscular dystrophy in humans. Ann Neurol 2005-02-01 [PMID: 15668942]
• Huang C, Zhou Q, Liang P et al. Characterization and in vivo functional analysis of splice variants of cypher. J Biol Chem 2003-02-01 [PMID: 12499364]

Bioinformatics

• Gene Symbol: LDB3
• Entrez:
  • 11155(Human)
• Uniprot:
  • NP_009009.1(Human)