LCMT1 Overexpression Lysate (Native)

Western Blot: LCMT1 Overexpression Lysate (Adult Normal) [NBL1-12460] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LCMT1.

• Reactivity: Hu
• Applications: WB

LCMT1 Overexpression Lysate (Native) Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC200018 and based on accession number NM_016309. The protein contains a C-terminal DDK tag.
• Specificity: Homo sapiens leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1, mRNA.
• Gene: LCMT1

Applications/Dilutions

• Application Notes: This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
• Theoretical MW: 38.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for LCMT1

• Type: Overexpression
• Protein State: Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for LCMT1 Overexpression Lysate (Native)

• CGI-68
• EC 2.1.1.-
• LCMT
• leucine carboxyl methyltransferase 1
• PPMT1
• protein phosphatase methyltransferase 1
• Protein-leucine O-methyltransferase

Background

LCMT1 catalyzes the methylation of the carboxyl group of the C-terminal leucine residue (leu309) of the catalytic subunit of protein phosphatase-2A (PPP2CA; MIM 176915) (De Baere et al., 1999 [PubMed 10600115]).[supplied by OMIM]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: LCMT1