PPME1 Antibody (OTI7F12)

Images

 
Western Blot: PPME1 Antibody (7F12) [NBP2-02240] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPME1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: PPME1 Antibody (7F12) [NBP2-02240] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PPME1.
Immunohistochemistry-Paraffin: PPME1 Antibody (7F12) [NBP2-02240] - Staining of paraffin-embedded Kidney tissue using anti-PPME1mouse monoclonal antibody.
Flow Cytometry: PPME1 Antibody (7F12) [NBP2-02240] - Analysis of Jurkat cells, using anti-PPME1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: PPME1 Antibody (7F12) [NBP2-02240] - Analysis of Hela cells, using anti-PPME1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI7F12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.96 mg/ml

Order Details

PPME1 Antibody (OTI7F12) Summary

Immunogen
Full length human recombinant protein of human PPME1 (NP_057231) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PPME1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2000
Theoretical MW
42.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.96 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PPME1 Antibody (OTI7F12)

  • EC 3.1.1
  • EC 3.1.1.-
  • FLJ22226
  • PME-1PME1
  • protein phosphatase methylesterase 1
  • protein phosphatase methylesterase-1

Background

Protein phosphatase methylesterase-1 catalyzes the demethylation of the protein phosphatase-2A catalytic subunit

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PPME1
Entrez