PPME1 Antibody (OTI7F12)

Western Blot: PPME1 Antibody (7F12) [NBP2-02240] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPME1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPME1.

Immunocytochemistry/Immunofluorescence: PPME1 Antibody (7F12) [NBP2-02240] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PPME1.

Immunohistochemistry-Paraffin: PPME1 Antibody (7F12) [NBP2-02240] - Staining of paraffin-embedded Kidney tissue using anti-PPME1mouse monoclonal antibody.

Flow Cytometry: PPME1 Antibody (7F12) [NBP2-02240] - Analysis of Jurkat cells, using anti-PPME1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry: PPME1 Antibody (7F12) [NBP2-02240] - Analysis of Hela cells, using anti-PPME1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI7F12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.96 mg/ml

PPME1 Antibody (OTI7F12) Summary

• Immunogen: Full length human recombinant protein of human PPME1 (NP_057231) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPME1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2000
• Theoretical MW: 42.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.96 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PPME1 Antibody (OTI7F12)

• EC 3.1.1
• EC 3.1.1.-
• FLJ22226
• PME-1PME1
• protein phosphatase methylesterase 1
• protein phosphatase methylesterase-1

Background

Protein phosphatase methylesterase-1 catalyzes the demethylation of the protein phosphatase-2A catalytic subunit

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPME1
• Entrez:
  • 51400(Human)