LATS2 Antibody

Product Discontinued

LATS2 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Large Tumor Suppressor 2 using the numbering given in entry NP_055387.1 (GeneID 26524).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LATS2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:1000-1:5000
• Control:

  HeLa Whole Cell Lysate
  LATS2 Overexpression Lysate

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 29317530).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LATS2 Antibody

• EC 2.7.11
• EC 2.7.11.1
• FLJ13161
• Kinase phosphorylated during mitosis protein
• KPM
• Large tumor suppressor homolog 2
• LATS (large tumor suppressor, Drosophila) homolog 2
• LATS, large tumor suppressor, homolog 2 (Drosophila)
• serine/threonine kinase KPM
• Serine/threonine-protein kinase kpm
• serine/threonine-protein kinase LATS2
• Warts-like kinase

Background

This gene encodes a serine/threonine protein kinase belonging to the LATS tumor suppressor family. The protein localizes to centrosomes during interphase, and early and late metaphase. It interacts with the centrosomal proteins aurora-A and ajuba and is required for accumulation of gamma-tubulin and spindle formation at the onset of mitosis. It also interacts with a negative regulator of p53 and may function in a positive feedback loop with p53 that responds to cytoskeleton damage. Additionally, it can function as a co-repressor of androgen-responsive gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LATS2 Antibody (NB200-199)(19)

Publications using NB200-199

• Wang X, Cai B, Yang X et al. Cholesterol Stabilizes TAZ in Hepatocytes to Promote Experimental Non-alcoholic Steatohepatitis Cell Metab. 2020-04-01 [PMID: 32259482]
• Ito T, Nakamura A, Tanaka I et al. CADM1 associates with Hippo pathway core kinases; membranous co-expression of CADM1 and LATS2 in lung tumors predicts good prognosis Cancer Sci. 2019-07-01 [PMID: 31069869] (WB, Human, Mouse)
• Rusnak L, Tang C, Qi Q et al. Large tumor suppressor 2, LATS2, activates JNK in a kinase-independent mechanism through ASK1 J Mol Cell Biol 2018-12-01 [PMID: 30496488] (IP, Human)
• Kedan A, Verma N, Saroha A et al. PYK2 negatively regulates the Hippo pathway in TNBC by stabilizing TAZ protein. Cell Death Dis 2018-09-24 [PMID: 30250159] (WB, Human)
• Wigerius M, Quinn D, Diab A et al. The polarity protein Angiomotin p130 controls dendritic spine maturation J. Cell Biol. 2018-02-05 [PMID: 29317530] (WB, Rat)
• Wang J, Sinnett-Smith J, Stevens JV et al. Biphasic regulation of Yes-associated Protein (YAP) cellular localization, phosphorylation and activity by G protein-coupled receptor agonists in intestinal epithelial cells: a novel role for protein kinase D (PKD) J. Biol. Chem. 2016-07-01 [PMID: 27369082] (WB, Human)
• Xiang L, Gilkes DM, Hu H et al. Hypoxia-inducible factor 1 mediates TAZ expression and nuclear localization to induce the breast cancer stem cell phenotype. Oncotarget. 2014-01-01 [PMID: 25587023] (IP, WB, Human)
• Pefani DE, Latusek R, Pires I et al. RASSF1A-LATS1 signalling stabilizes replication forks by restricting CDK2-mediated phosphorylation of BRCA2. Nat. Cell Biol. 2014-09-14 [PMID: 25218637]
• Reuven N, Adler J, Meltser V, Shaul Y. The Hippo pathway kinase Lats2 prevents DNA damage-induced apoptosis through inhibition of the tyrosine kinase c-Abl. Cell Death Differ 2013-07-12 [PMID: 23852372] (WB, Human)
• Zhang L, Iyer J, Chowdhury A et al. KIBRA regulates aurora kinase activity and is required for precise chromosome alignment during mitosis. J Biol Chem. 2012-10-05 [PMID: 22904328]
• Vigneron AM, Vousden KH. An indirect role for ASPP1 in limiting p53-dependent p21 expression and cellular senescence. The EMBO Journal. 2011-11-08 [PMID: 22068052]
• Zhang H, Deo M, Thompson RC et al. Negative regulation of Yap during neuronal differentiation. Dev Biol 2012-01-01 [PMID: 22037235]
• Zhang K, Rodriguez-Aznar E, Yabuta N et al. Lats2 kinase potentiates Snail1 activity by promoting nuclear retention upon phosphorylation. EMBO J 2011-09-01 [PMID: 21952048]
• Paramasivam M, Sarkeshik A, Yates JR 3rd et al. Angiomotin family proteins are novel activators of the LATS2 kinase tumor suppressor. Mol Biol Cell 2011-10-01 [PMID: 21832154]
• Go YM, Kang SM, Roede JR et al. Increased inflammatory signaling and lethality of influenza H1N1 by nuclear thioredoxin-1. PLoS One 2011-01-01 [PMID: 21526215]
• Tschop K, Conery AR, Litovchick L et al. A kinase shRNA screen links LATS2 and the pRB tumor suppressor. Genes Dev 2011-04-01 [PMID: 21498571]
• Murakami H, Mizuno T, Taniguchi T et al. LATS2 Is a Tumor Suppressor Gene of Malignant Mesothelioma. Cancer Res. 2011-01-18 [PMID: 21245096] (WB, Human)
• Benhamouche S, Curto M, Saotome I et al. Nf2/Merlin controls progenitor homeostasis and tumorigenesis in the liver. Genes Dev 2010-08-01 [PMID: 20675406]
• Kilili GK, Kyriakis JM. Mammalian Ste20-like kinase (Mst2) indirectly supports Raf-1/ERK pathway activity via maintenance of protein phosphatase-2A catalytic subunit levels and consequent suppression of inhibitory Raf-1 phosphorylation. J Biol Chem 2010-05-01 [PMID: 20212043]

Bioinformatics

• Gene Symbol: LATS2
• Entrez:
  • 26524(Human)
• Uniprot:
  • Q9NRM7(Human)